An experimental study on protective effect of meglumine adenosine cyclosphosp on cerebral ischemia reperfusion injury in rabbits

Abstract

Objective To investigate the protective effect of meglumine adenosine cyclosphosp (MAC) on the cerebral ischemia-reperfusion (I/R) injury in rabbits. Methods Twenty four healthy rabbits were randomly divided into control group (n=6), I/R group (n=6), MAC pretreated group (n=6), and MAC treated group (n=6). Cerebral ischemia-reperfusion injury model was made by separating and electrocoagulating vertebral arteries and clipping common carotid arteries in the latter 3 groups after anesthesia. The sham-operated group underwent vessel separation without clipping. I/R group was administered with nothing, while MAC pretreated group with MAC before ischemia, and MAC treated group with MAC just after ischemia. Blood was gathered from jugular vein before ischemia, and 30 min, 1 h, and 2 h after reperfusion for testing IL-8, superoxide dismutase (SOD) and malondialdehyde (MDA). The brain tissue slice was observed by optical microscope. Results Compared to control group and before ischemia, the levels of IL-8 and SOD in serum were significantly increased and decreased, and the levels of MDA was significantly increased at 30 min after reperfusion in I/R group; the levels of IL-8 and MDA in serum were significantly increased, and the levels of SOD in serum was significantly decreased at 1 h and 2 h after reperfusion in I/R group. The levels of IL-8 in serum was less at 30 min and 1 h and 2 h after reperfusion in MAC pretreated group than in I/R group.At 1 h and 2 h after reperfusion, the levels of MDA in serum was less and the levels of SOD in serum was higher in MAC pretreated group than in I/R group. At 1 h and 2 h after reperfusion, the levels of IL-8 in serum were less and the levels of SOD in serum were higher in MAC treated group than in I/R group. The levels of MDA in serum were less at 2 h after reperfusion in MAC treated group than in I/R group. Compared to I/R group, pathological change was lighter in the MAC pretreated and MAC treated group. Conclusions MAC has a fine cerebral-protective effect and has no side effect. Key words: Cyclic AMP/PD; Brain ischemia/DT; Reperfusion injury/DT