Abstract
BackgroundThe study aimed to investigate whether and how general and partial time intervals between processes, from human chorionic gonadotrophin (HCG) trigger to intracytoplasmic sperm injection (ICSI), affected the laboratory and reproductive outcomes in ICSI cycles.MethodsThis was a retrospective data analysis of 3602 women who underwent ICSI treatment cycles using partner or donor sperms, performed at Reproduction Medicine Center of Tongji Hospital of Tongji Medical College of Huazhong University of Science and Technology (Wuhan, China) between October 2016 and September 2018. The clinical pregnancy rate was the major outcome in the study. The fertilization and available embryo rates were secondary outcomes.ResultsData from 3602 consecutive fresh ICSI cycles was analysed. Multivariate linear regression and logistic regression analysis of factors related to fertilization and clinical pregnancy rates showed that fertilization rate (P = 0.001) and clinical pregnancy rate (P = 0.037) were significantly associated with denudation (DN)-ICSI interval. Long DN-ICSI interval was associated with higher rate of fertilization than short DN-ICSI interval but significantly decreased clinical pregnancy rate when the interval is over 4 h (P < 0.05).ConclusionsDN-ICSI time interval can act as an independent predictor for clinical outcomes in ICSI cycles. The optimal time for ICSI is within 4 h after oocyte denudation for excellent laboratory and reproductive outcomes in ICSI cycles.
Highlights
The study aimed to investigate whether and how general and partial time intervals between processes, from human chorionic gonadotrophin (HCG) trigger to intracytoplasmic sperm injection (ICSI), affected the laboratory and reproductive outcomes in ICSI cycles
In order to obtain an optimal clinical outcome in ICSI cycles, we investigated the large cohort of successive ICSI instances to explore whether and how general and partial time intervals between processes, from human chorionic gonadotrophin (HCG) trigger to sperm microinjection recorded by the operator affected the laboratory and clinical outcomes in assisted reproductive technology (ART) treatments
Our present study discovered DN-ICSI time interval acted as an independent predictor for clinical outcomes in ICSI cycles
Summary
The study aimed to investigate whether and how general and partial time intervals between processes, from human chorionic gonadotrophin (HCG) trigger to intracytoplasmic sperm injection (ICSI), affected the laboratory and reproductive outcomes in ICSI cycles. In order to get more successful ICSI outcomes, the operation and its optimal timings including oocyte pick up (OPU), denudation (DN) and ICSI in ICSI procedures have been investigated [1, 2]. The viewpoint finds general acceptance that DN and ICSI should be performed for a while after OPU in order to get sufficient time for achieving the maturation procedure [1]. Satoshi Mizuno et al [1] showed the presence of intact cumulus cells during the preincubation period for ICSI should be considered as a critical factor in fertilization and embryonic development, oocytes needed
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