Performance of the Pseudomonas chlororaphis biocontrol agent MA 342 against cereal seed-borne diseases in field experiments

Abstract

The bacterial biocontrol agent Pseudomonas chlororaphis, strain MA 342, was tested for activity against a number of cereal seed-borne diseases in a total of 105 field experiments carried out at different locations in Sweden during the years 1991-1996. Bacterial liquid culture was directly applied to pathogen-infested seeds of barley, oats, wheat and rye without additives. The seeds were then dried and sown in field together with fungicide-treated and untreated seeds used as controls. The bacterization controlled seed-borne diseases caused by Drechslera (Pyrenophora) graminea, D. teres, D. avenae, Ustilago avenae, U. hordei, and Tilletia caries, as effectively as guazatine + imazalil, and these effects were consistent over the years and over varying climatic zones. Diseases caused by pathogens like U. nuda, soil-borne T. caries and T. contraversa were not controlled and the bacterization gave less than full effect against diseases caused by Microdochium (Fusarium) nivale, and Bipolaris sorokiniana (Cochliobolus sativus). Bacterized seeds could be stored dry for at least two years without losing the disease suppressing effect of the bacterial treatment, when tested in the field.