Abstract
Current and future research focuses on the use of renewable technologies and materials to stabilise weak soils, of varying degrees, for road construction applications. Soil stabilisation is a method of strengthening a natural soil to meet this purpose. Our interest is in the use of bio-based components, derived from microbial growth processes, that contribute to the needed desirable strength characteristics for in situ soil stabilisation. This investigation focuses on novel Bacillus-based stabilisers obtained from the vegetative and spore growth stage. In this study, eighteen bio-based components were derived from a Bacillus licheniformis fermentation and extracted into various aqueous and non-aqueous fractions for strength property assessment. The strength properties of the treated soils (i.e., dolerite and weathered granite soil) were assessed via previously developed lab-scale equipment to rapidly pre-select the best performing fractions, (i.e., compression stress, erosion, abrasion, and water absorption tests). The effect of one superior performing prototype (a) was validated at large-scale, using standard erosion and abrasion tests (i.e., whole broth at 1.8% stabiliser concentrations), and showed resistance to abrasion (3.37 ± 0.03%) (p value ≤ 0.0001) and resistance to erosion (33.20 ± 0.15%) (p value ≤ 0.001). The elemental composition and microstructure of the bio-stabilised soil was determined using energy dispersive X-ray spectroscopy and scanning electron microscopy, respectively. This evaluation formed part of the selection of the best performing Bacillus derived fractions and achieved a proof of concept for the next phase of product prototype development. This study demonstrated a novel bio-mediated approach to the overall criteria for evaluation and selection of candidate product prototype/s, for stabilisation of two varying soils, and for potential application in road construction works.
Highlights
The results obtained from this study demonstrated an assessment process of the eighteen-potential bio-based stabilisers
The strength tests have proven to be important factors to determine the effectiveness of soil particle reinforcement
Out of eighteen fractions, six proved to have superior strength and durability properties based on the key measurements
Summary
South Africa has more than 500,000 km of unpaved roads; these roads generally do not provide all-weather pass-ability, they are of poor riding quality, impassable in wet weather, and produce excessive dust in the dry weather. These roads are critical in providing access to rural and poorer areas, where the majority of the population reside. These roads are an important factor in supporting a rural-based economy of the country
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
