Abstract
A knowledge of the relationships between particle size and layer thickness on the one hand and migration distance and plate height on the other has prompted the development of the pre-coated HPTLC plate for “nano-TLC”. A separation performance which is very high with respect to time is achieved with a migration distance of 3–7 cm with a plate height of about 12 μm and several thousand theoretical plates, thanks to the possibility of simultaneous chromatography. In HPTLC quantitative evaluation, standardised chromatographic conditions are essential. In this context the complex influences of different types of chamber and solvents on linear and circular chromatography are described. Various methods of conditioning the adsorbent by immersion impregnation, as well as detection procedures are discussed. The scope for varying TLC procedures and the advantages of TLC in general are listed.
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