Abstract
Publisher Summary This chapter reviews the development of high performance thin layer chromatography (HPTLC). The manufacture of high quality sorbents for thin-layer chromatography requires a production plant and equipment set up according to the latest technological advances and continuous, multiplecheck supervision. Carefully selected standardized pre-coated TLC preparations in well-defined chromatographic systems are the best guarantee for reproducible results both in qualitative and quantitative terms. The chromatographic characteristics are influenced by the type of chamber used and the activity of the adsorbent. The increase in Rf-values with higher humidity figures in the Vario-KS-chamber is produced by the selectively higher inactivation of the sorbent. The slight increase in plate height in the narrow chamber as compared to the normal chamber with saturated atmosphere cannot be explained satisfactorily. There might be a connection between this phenomenon and the somewhat longer migration time.
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