Abstract
AbstractWe synthesized a novel polymer‐based stationary phase modified by N‐methyl‐d‐glucamine. Although there is a tertiary amine group on the ligand of the special stationary phase, the performance of protein separation is cation exchange chromatography, instead of anion exchange chromatography. The similar retention behavior was also observed in ion exchange chromatography mode of two similar stationary phases and a commercial hydrophobic interaction chromatography column, which have no negatively charged groups. It could be caused by the hydrogen‐bonding interaction between chloride ions and hydroxyl groups.
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