Abstract
Aiming at evaluating the electrophoresis profile of serum and peritoneal protein in horses submitted to an experimental model of intestinal obstruction, 24 animals were divided into four groups: instrumented control (GI), duodenum obstruction (GII), ileum obstruction (GIII) and large colon obstruction (GIV). Blood and peritoneal fluid samples were collected before the surgical procedures (T0), 3h after the obstruction (Ti) and 1, 3, 12, 24, 72, 120 and 168h after reperfusion (Tr). After sample centrifuging and fractioning, total protein were determined by biuret method, and the proteic fractions were separated by electrophoresis in agarose gel. Alterations were observed in serum and peritoneal concentrations of α, β and γ globulins on the studied animals. The changes occurred due to the intestinal inflammatory process caused by the obstruction model and the surgical trauma, associated to laparotomy. Animals submitted to duodenum and ileum obstruction showed higher serum and peritoneal concentrations of acute phase proteins. The electrophoretic separation of proteins included in the peritoneal liquid is more sensible when compared to the serum concentration to the diagnosis of abdominal inflammatory process. KEYWORDS: Acute phase protein, colic, horse.
Highlights
Aiming at evaluating the electrophoresis profile of serum and peritoneal protein in horses submitted to an experimental model of intestinal obstruction, 24 animals were divided into four groups: instrumented control (GI), duodenum obstruction (GII), ileum obstruction (GIII) and large colon obstruction (GIV)
As proteínas de fase aguda (PFA) possuem a finalidade de inibir a continuidade do dano tecidual, isolando e destruindo o organismo agressor e ativando o processo de reparação necessária para o retorno do organismo às funções normais (CERÓN et al, 2005)
Diferindo dos resultados obtidos por CARAPETO et al (2006), em equinos com cólica em que os maiores percentuais de elevação dos teores séricos das proteínas proteínas de fase aguda foram associados a sinais de endotoxemia, como a hipertermia, depressão, dor abdominal, anorexia, leucopenia, e à presença neutrófilos tóxicos (citoplasma basofílico e vacuolização), os resultados do proteinograma sérico e peritoneal apresentados pelos animais dos quatro grupos experimentais não foram associados a alterações clínicas e/ou à presença de intercorrências no pós-operatório
Summary
Aiming at evaluating the electrophoresis profile of serum and peritoneal protein in horses submitted to an experimental model of intestinal obstruction, 24 animals were divided into four groups: instrumented control (GI), duodenum obstruction (GII), ileum obstruction (GIII) and large colon obstruction (GIV). Ensaio realizado por SAQUETTI et al (2008) em equinos submetidos à obstrução experimental do cólon menor revelou aumento na concentração proteí na no líquido peritoneal e, segundo estes autores, os resultados deveram-se não somente à lesão intestinal desencadeada pela obstrução experimental como também ao trauma cirúrgico.
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