Abstract
Wounds have slowed healing due to the formation of biofilms, which are the surface formation of bacterial cells that are covered by the polymeric substance. Staphylococcus aureus is a gram-positive bacteria that play a role in producingmultilayer in biofilms and is very easy to resistant to antibiotics. VCO has the ability as ana anti-bacterial content based on lauric acid and kaprat acid. This study used experimental Quasy with a mixed Methode method. VCO is made with 3 methods, fishing, heating and mixing on physicochemical test. Physicochemical test results show, the highest water content is found in the VCO heating, the same three-type weight, the highest peroxide number is in the VCO mixing and the highest free fatty acids on the VCO fishing. The results of antibacterial test with Kirby-Baur method There is no barrier zone from the 3 types of VCO. VCO used as a topical is VCO fishing.The results of a test analysis of the results of observations in patients as much as 15 people for 9 days in 3 treatments with chronic wounds obtained p < 0.05 (0.000) which means during the treatment that the wound condition is better. Keyword : Organoleptic, Staphylococcus aureus, Physicochemical, VCO
Highlights
The results of a test analysis of the results of observations in patients as much as 15 people for 9 days in 3 treatments with chronic wounds obtained p < 0.05 (0.000) which means during the treatment that the wound condition is better
Wounds have slowed healing due to the formation of biofilms, which are the surface formation of bacterial cells that are covered by the polymeric substance
Staphylococcus aureus is a gram-positive bacteria that play a role in producing multilayer in biofilms and is very easy to resistant to antibiotics
Summary
Bilangan peroksida 0.41 peroksida 0.19 peroksida mgrek O2/Kg mgrek O2/Kg 1.01 mgrek. Dari penjabaran tabel 1 diatas dapat dijelaskan bahwa kandungan kadar air tertinggi dimiliki oleh VCO dari hasil pemenasan 1.4 % untuk berat jenisnya ketiganya tidak menunjukkan perbedaan yang signifikan, namun yang tertinggi adalah VCO pemanasan. Perbedaan warna yang signifikan antara tiga sampel A VCO pancingan sampel VCO pemanasan sampel VCO mixing (p=0.000 < α = 0,05). Endapan tidak menunjukkan perbedaan antara 3 sampel dengan hasil (p=0.90 > α = 0,05). Uji zona hambat pertumbuhan staphylococcus aureus dengan menggunakan metode Kirby-baur disk diffusion. 1 sampel control negative dan 3 sampel untuk jenis VCO dengan metode yang berbeda. Jenis bakteri yang digunakan pada uji ini adalah staphylococcus aureus Hasil yang di dapakan dari hasil uji Kirby-baur disk diffusion control positif menunjukkan memilki zona hambat, control negative tidak terjadi zona hambat dan ketiga jenis varian VCO menunjukkan hasil 0 (nol) yang artinya tidak memilki zona hambat. Hasil uji antibakteria dengan melihat zona hambat bakteri menujukkan bahwa dari lima sampel yang digunakan hanya sampel kontrol possitif yang menunjukkan adanya zona hambat pada uji tersebut
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