PERBANDINGAN EFEKTIVITAS ANTIBAKTERI EKSTRAK DAUN SEMBUNG RAMBAT (MIKANIA MICRANTHA) DAN NaOCl 0,5% TERHADAP PERTUMBUHAN BAKTERI ENTEROCOCCUS FAECALIS SECARA IN VITRO

Abstract

Enterococcus faecalis is a species that often found in root canal infection. One of the stage in root canal treatment which can relieve the bacteria is irrigation. Nowadays, sodium hypochlorite (NaOCl) is one of the most used material in irrigation, but it can caused an irritation if periradicular tissue is pushed over. Mile-a-minute (Mikania micrantha) is a natural ingredient containing the active substance such as secondary metabolites that act as an antibacterial. The purpose of this research was to know the comparison of antibacterial effectivity between mile-a-minute leaf extract (Mikania micrantha) and 0,5% NaOCl to Enterococcus faecalis growth as in vitro ways. The method of this research was experimental laboratories with posttest only control group design. Research of 2%, 1%, 0,5%, 0,25%, 0,125% concentration of mile-a-minute leaf extract and 0,5% NaOCl using disc diffusion method on Mueller-Hinton Agar (MHA) medium and the formed inhibit zone was measured with sliding caliper. The data was analyzed by Kruskal Wallis and Mann-Whithney test. The result of this research found that 2%, 1%, 0,5%, 0,25%, 0,125% concentration of mile-a-minute leaf extract and 0,5% NaOCl form an inhibit zone. Kruskal Wallis test showed that there were significant difference among the groups (p <0,05). The conclusion of this research was mile-a-minute leaf extract was more effective in inhibiting the growth of Enterococcus faecalis than 0,5% NaOCl.