Abstract
Rabies is an infectious disease, zoonotic, caused by virus from the genus Lyssa virus and generally transmitted by the bite of rabid animal, especially rabies infected dog. Rabies is preventable but is always fatal to humans if the central nervous system (CNS) is infected. Vaccination has been used as one of rabies prevention programmed. A total of 83 samples were tested using an Indirect ELISA (Enzyme Linked Immunosorbent Assay) to identify post-vaccination rabies antibody titer. Antibody titres correlated with absorbance values and standard solutions concentrations. Absorbance value can be determined using ELISA reader and mobile phone camera. Absorbance were read at 450 nm and 620 nm as reference using ELISA reader and image from mobile phone camera using image processing software (ImageJ). The aim of this study is to compared between ELISA reader as gold standard and mobile phone camera through validity testing such sensitivity, specificity, and accuracy. There is no significant difference between gold standard and alternative test equipment. The mobile phone camera has sensitivity 98,6%, specificity 88.8 % and accuracy 97,5%. The image processing method using ELISA reader is relatively expensive and difficult to hold in laboratory with minimum funds. Image processing method using a mobile phone camera with ImageJ application is expected to be an alternative tool to read the result of ELISA.
Highlights
Jurnal Veteriner diadakan di laboratorium dengan dana minimum sehingga metode pengolahan citra menggunakan kamera telepon genggam dengan aplikasi ImageJ diharapkan mampu menjadi alternatif pilihan untuk alat pembaca hasil EnzymeLinked Imunosorbent Assay (ELISA) (Enzyme-Linked Immunosorbent Assay)
The aim of this study is to compared between ELISA reader as gold standard and mobile phone camera through validity testing such sensitivity, specificity, and accuracy
There is no significant difference between gold standard and alternative test equipment
Summary
Sampel darah diambil dari vena cephalica antebrachii dan disimpan di cool box berisi es batu. Serum diambil dari sampel darah dan dimasukkan ke microtube untuk pengujian selanjutnya. Pembacaan hasil ELISA menggunakan ELISA reader dengan dua filter yaitu panjang gelombang 450 nm dan 620 nm sebagai reference. Hasil dari pembacaan menggunakan ELISA reader berupa nilai (Optical Density) atau absorbansi. Sumbu y merupakan nilai absorbansi dan sumbu x merupakan konsentrasi larutan standar yang telah diperoleh yaitu 1,6; 0,8; 0,4; dan 0,2 (Equivalent Unit). Nilai a dan b adalah konstanta yang diperoleh dari perhitungan intercept dan slope dan dapat digunakan sebagai acuan dalam perhitungan titer antibodi sampel dengan rumus x= y-a/b. Dalam hal ini A merupakan nilai absorbansi, I adalah intensitas masing-masing warna merah, hijau dan biru serta Io merupakan nilai maksimal dari sebuah pixel yaitu 255 (Soldat et al, 2009). Selanjutnya, nilai absorbansi dan konsentrasi disajikan dalam bentuk kurva standar/kalibrasi sehingga diperoleh persamaan garis linear y=a+bx.
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