Abstract
Sodium hypochlorite (NaOCl) is a powerful disinfectant used in root canal therapy, yet it has some disadvantages. There are growing interests of using extracts of natural materials containing antibacterial secondary metabolite compounds as an alternative of irrigation solution. Aim, to compare the antibacterial activity of white turmeric extracts and 2,5% sodium hypochlorite irrigant against Enterococcus faecalis. Methods, this was a quasi experimental study. White turmeric extracts at the concentrations of 75%, 50%, 25% and 10% were used as experimental groups, while 2,5% NaOCl and distilled water were the control groups. Suspensions of Enterococcus faecalis were prepared to a 0.5 Mc Farland standard and applied onto petri plates containing Muller Hinton Agar. Six wells were created in each plate, and filled with 20 mL of the test solution. Plates were incubated at 37 ° C for 24 hours. The antibacterial activity was evaluated by measuring the clear zone around the well. Data were analyzed by Kruskall Wallis and continued by Mann U Whitney. Results, the largest growth inhibition zones were produced when the test bacteria were in contact with 2,5% NaOCl (15,0233 mm). White turmeric extract 75% concentration had weak antibacterial effect, while white turmeric extract 50%, 25%, 10% concentration, and distilled water did not has antibacterial properties against Enterococcus faecalis. Conclusion, white turmeric extract 75% concentration can inhibit the growth of Enterococcus faecalis with weak antibacterial activity.
Highlights
a powerful disinfectant used in root canal therapy
growing interests of using extracts of natural materials containing antibacterial secondary metabolite compounds as an alternative of irrigation solution
10% were used as experimental groups
Summary
Sebanyak 500 gram rimpang temu putih dicuci dengan air mengalir dan diiris tipistipis (± 3 mm), kemudian dikeringkan menggunakan oven dengan suhu 50°C semalaman sampai rimpang kering yang ditandai dengan mudah dipatahkan atau hancur. Penyaringan dengan corong saring dilakukan untuk mendapatkan ekstrak dalam bentuk cair, kemudian diuapkan sampai bebas dari pelarut etanol menggunakan rotary evaporator pada suhu 40°C selama 3 jam hingga diperoleh ekstrak kental dengan konsentrasi 100%.13. Bakteri Enterococcus faecalis yang telah disuspensikan diambil dengan syringe 1cc sebanyak 0,5 ml, kemudian dituangkan ke dalam cawan petri yang berisi media MHA steril dan dioleskan secara merata. Lubang-lubang ini masing-masing diisi dengan larutan ekstrak rimpang kunyit putih konsentrasi 75%, 50%, 25%, dan 10%, NaOCl 2,5% dan aquades sebagai sebanyak 5 μl dengan menggunakan mikropipet. Cawan petri kemudian dibiarkan dalam suasana anaerob dan diinkubasi dengan suhu 37oC selama 24 jam.[14] Pengukuran Zona Hambat. Pengamatan dilakukan dengan mengukur diameter vertikal, horizontal, dan diagonal pada zona bening (clear zone) di sekitar sumuran menggunakan jangka sorong dengan ketelitian 0.01 mm sebanyak 3 kali untuk menghindari kesalahan pengukuran.
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