Abstract
Simple SummaryThe virulence factors and pathogenesis of S. suis are inconclusive. Here, the associated proteins, or their derived peptides, involved in the survival of S. suis when simulated with a blood environment are demonstrated. The results reveal the derived peptides or proteins of S. suis potentially serving as the putative virulence factors. Further studies based on our findings could be used to fulfill the knowledge gap of S. suis pathogenesis.Streptococcus suis (S. suis) is a zoonotic pathogen causing severe streptococcal disease worldwide. S. suis infections in pigs and humans are frequently associated with the virulent S. suis serotype 2 (SS2). Though various virulence factors of S. suis have been proposed, most of them were not essentially accounted for in the experimental infections. In the present study, we compared the peptidomes of highly virulent SS2 and SS14 in humans, the swine causative serotypes SS7 and SS9, and the rarely reported serotypes SS25 and SS27, and they were cultured in a specified culture medium containing whole blood to simulate their natural host environment. LC-MS/MS could identify 22 unique peptides expressed in the six S. suis serotypes. Under the host-simulated environment, peptides from the ABC-type phosphate transport system (SSU05_1106) and 30S ribosomal protein S2 (rpsB) were detected in the peptidome of virulent SS2 and SS14. Therefore, we suggest that these two proteins or their derived peptides might be involved in the survival of S. suis when simulated with a blood environment.
Highlights
Among 35 serotypes classified by the immunogenics of their capsular polysaccharides [1], SS serotype 2 (SS2) is the most prevalent, followed by SS14, the causative agent of S. suis zoonotic infection in humans and pigs worldwide [2,3]
A high-performance liquid chromatography-mass spectrometry/mass spectrometry method (LC-Mass spectra (MS)/MS) was used to determine the peptidomes of the highly virulent to humans SS2 and SS14, swine causative serotypes SS7 and SS9, and rarely reported serotypes SS25 and SS27 cultured in a specified culture medium containing whole blood, simulating their natural host environment
S. suis expressed its virulence factor in the form of proteins or peptides to survive in the host
Summary
Streptococcus suis is a zoonotic bacterium causing severe streptococcal infection in both humans and pigs. Among 35 serotypes classified by the immunogenics of their capsular polysaccharides [1], SS serotype 2 (SS2) is the most prevalent, followed by SS14, the causative agent of S. suis zoonotic infection in humans and pigs worldwide [2,3]. A high-performance liquid chromatography-mass spectrometry/mass spectrometry method (LC-MS/MS) was used to determine the peptidomes of the highly virulent to humans SS2 and SS14, swine causative serotypes SS7 and SS9, and rarely reported serotypes SS25 and SS27 cultured in a specified culture medium containing whole blood, simulating their natural host environment. This study aimed to explore some putative virulence factors involved in the survival of S. suis in the blood environment
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