Abstract
Peptides were separated by gel filtration high-performance liquid chromatography with gradient elution. Two gradient elution systems were applied: (1) 0 to 20% acetonitrile in 30 m M phosphate buffer, pH 8.0 and (2) 30 m M phosphate buffer, pH 8.0 to water; with the polymer-type gel filtration column we used, excellent separation of tryptic peptides from hen egg-white lysozyme was achieved within 50 min. System 2 was applied to a separation of V8 protease peptide from yam tuber chitinase and yielded two to three times higher amounts of peptides than reversed-phase high-performance liquid chromatography.
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