Abstract
The Geminiviruses genome is a small, single strand DNA that replicates in the plant cell nucleus. Analogous to animal DNA viruses, Geminiviruses depend on the host replication machinery to amplify their genomes and only supply the factors required to initiate their replication. Consequently, Geminiviruses remove the cell-cycle arrest and induce the host replication machinery using an endocycle process. They encode proteins, such as the conserved replication-associated proteins (Rep) that interact with retinoblastoma-like proteins in plants and alter the cell division cycle in yeasts. Therefore, the aim of this work is to analyze the impact of Pepper Golden Mosaic Virus (PepGMV) Rep protein in mammalian cells. Results indicate that the pTracer-SV40:Rep construction obtained in this work can be used to analyze the Rep protein effect in mammalian cells in order to compare the cell cycle regulation mechanisms in plants and animals.
Highlights
Geminiviruses are plant pathogens which have small, single strand DNA genomes
One corresponds to the linearized pTracerSV40 vector (Figure 1b, lines 2–4) which has a size of 4.2 kb and a second band corresponding to replication-associated proteins (Rep) (Figure 1b, lines 2–4)
The Pepper Golden Mosaic Virus Rep gene was expressed in mammalian cells
Summary
Geminiviruses are plant pathogens which have small, single strand DNA genomes. They are characterized by their unique geminate capsids formed by two incomplete icosahedra [1,2].Geminiviruses constitute a large family that is divided into the Begomovirus, Mastrevirus, Curtovirus, and Topocuvirus genera based on genome arrangement, insect vector, and host range [3,4]. Geminiviruses are plant pathogens which have small, single strand DNA genomes. They are characterized by their unique geminate capsids formed by two incomplete icosahedra [1,2]. Geminiviruses constitute a large family that is divided into the Begomovirus, Mastrevirus, Curtovirus, and Topocuvirus genera based on genome arrangement, insect vector, and host range [3,4]. The. Begomovirus genus is the largest and includes whitefly-transmited, and bipartite (genome divided into two components) geminivirus [5]. Pepper Golden Mosaic Virus (PepGMV) belongs to Begomovirus genus and naturally infects pepper, tomato, tobacco, and other solanaceous crops. The typical symptoms induced by PepGMV in pepper plants, are yellow mosaic and wrinkled leaf and stunting of the plant [6]
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