Abstract
The chitosanase producing strain Penicillium janthinellum D4, was isolated from soil in Tay Nguyen University in Vietnam. Two chitosanases F1 F2 were purified from the culture supernatant by ammonium sulfate precipitation, DEAE-Sepharose column chromatography and Macro-prep DEAE Cartridge. The molecular mass of CHS was estimated to be 49kDa by SDS-PAGE. The optimum temperature, optimum pH, thermal stability, and pH stability of CHS F2 were 60℃, pH 7~9, 25~40℃, and pH 10, respectively. The activity of CHS F2 was inhibited by Fe2+,Cu2+,and Mn2+.
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