Abstract
The chitosanase, chitinase and proease-producing strain TKU030 was isolated from soil in Tamkang University with squid pen powder as the sole carbon/nitrogen source and identified as Bacillus cereus. A chitosanase (CHS) was purified from the culture supernatant by ammonium sulfate precipitation, Sephacryl S-100 gel chromatography, DEAE- Sepharose column chromatography and Sephacryl S-100 rechromatography . The molecular mass of CHS determined by SDS-PAGE was approximately 43 kDa. The optimum temperature, optimum pH, thermal stability, and pH stability of CHS were 40oC, pH 4, 30-40oC and pH 3-7, respectively.The activity of CHS was inhibited by Mn2+, Cu2+, Fe2+ and a boron-containing compound of TKUPSP017. The inhibition of CHS activity reached 100% at 0.05% (w/v) of TKUPSP017. The inhibitory activity increased with the preincubating time of TKUPSP017 and CHS. The effects of inhibitor structures on enzyme activity and growth of strain TKU030 in media supplemented with TKUPSP017 were also studied.Growth of strain TKU030 was not influenced by inhibiting CHS activity but enhanced by increasing TKUPSP017 concentration.
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