Abstract
Penicillin G reacts with 2,4-pentanedione to produce a derivative, which, in turn, reacts with Ehrlich's aldehyde reagent. The colored reaction product describes maximum absorbance in the region of 545–560 nm. Since maximum absorbance of the Ehrlich's complex of the pentanedione derivative of δ-aminolevulinic acid (ALA) is within this region, the excretion of penicillin G in urine will generate erroneously high ALA values.
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