Abstract
Mycobacterial culture provides definitive diagnosis of tuberculosis (TB), but commercially readyto-use culture media for Mycobacterium bovis are rarely available. The aims of this study were todevelop and to evaluate the ability of M. Bovis to grow in Modified Ogawa Agar (MOA) in comparisonwith the available culture media, such as Lowenstein Jensen (LJ) and Modified Ogawa (MO). Eachmedia were inculation with 0.1 ml suspension of 105 CFU/mL M. bovis and M. phlei in PhosphateBuffer Saline (PBS) and each media was replicated in five tubes. Mycobacterium phlei grew in everymedium since day 4. M. bovis grew in media LJ and MO since day 17, but failed to grow in mediumMOA. The recovery rate of M. phlei in LJ and MOA were significantly different. The ability of MOA tocultivate M. phlei was different from LJ. Colonies of M. phlei in MOA were easier to be harvested, muchsimpler to prepare, and more feasible than medium LJ. The recovery rate of M. bovis in media LJ andMO were not significantly different, but medium MO were much simpler to prepare and more feasiblethan medium LJ. Media MOA were able to cultivate M. phlei, but proven unable to cultivate M. bovisin this research.
Highlights
Tuberkulosis (TB), merupakan penyakit infeksius yang tersebar luas di seluruh dunia, disebabkan oleh infeksi organisme yang merupakan anggota Mycobacterium tuberculosis complex (MTBC, tuberkel basili mamalia)
Each media were inculation with 0.1 ml suspension of 105 colony forming unit (CFU)/mL M. bovis and M. phlei in Phosphate Buffer Saline (PBS) and each media was replicated in five tubes
Mycobacterium phlei grew in every medium since day 4
Summary
Tuberkulosis (TB), merupakan penyakit infeksius yang tersebar luas di seluruh dunia, disebabkan oleh infeksi organisme yang merupakan anggota Mycobacterium tuberculosis complex (MTBC, tuberkel basili mamalia). Bakteri MTBC terdiri atas kelompok mikobakteri yang sangat erat hubungannya, meliputi beberapa spesies, yaitu M. tuberculosis, M. bovis, M. africanum, M. canetti, M. pinnipedii, M. caprae, dan M. microti. Akibat kurangnya kemampuan laboratorium dalam mengisolasi dan membedakan organisme ini dari bakteri MTBC lainnya, maka kasuskasus TB yang disebabkan oleh M. bovis pada kebanyakan negara berkembang tidak diketahui, meskipun tampaknya lebih tinggi dibandingkan dengan di negara-negara industri (Cosivi et al, 1998; Michel et al, 2010). Media ideal untuk isolasi tuberkel basili harus (a) ekonomis dan mudah dibuat, (b) menghambat pertumbuhan kontaminan, (c) mendorong mikobakteri yang jumlahnya hanya sedikit dapat tumbuh dengan subur, dan (d) memungkinkan melakukan deferensiasi isolat secara dini berdasarkan morfologi koloni (Weyer et al, 1998b). Media yang dapat menumbuhkan M. tuberculosis adalah Löwenstein Jensen (LJ) yang mengandung telur dan diperkaya dengan gliserol dan asparagin, serta media agar maupun cair yang diberi serum atau albumin sapi. Penelitian ini bertujuan untuk mengembangkan dan menilai kemampuan media Modifikasi Ogawa Agar (MOA) untuk menumbuhkan M. bovis, dibandingkan dengan media yang digunakan saat ini, yaitu Löwenstein Jensen (LJ) dan Modifikasi Ogawa (MO)
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