Pengembangan Epitop PMSA1 Plasmodium falciparum Isolat Lokal Sikka Endemik Malaria

Abstract

The prevalence of malaria in East Nusa Tenggara Province is relatively high, one of the causes is the genomic polymorphism of Plasmodium Merozoite Surface Antigen 1 (PMSA1) on plasmodium that invades erythrocytes. The development of PMSA1 epitopes for Sikka isolates has not been widely reported, so it is necessary to identify PMSA1 antigens and their epitopes in depth for the design of malaria vaccines. Parasite examination through a microscope, genomic DNA of P. falciparum was isolated then the gene encoding PMSA1 was PCR amplified and cloned using pGEM-Teasy and E. coli TOP'10. Target DNA was confirmed by PCR colony and sequencing, phylogenetic tree construction, and epitope analysis. A total of 15 isolates of genomic DNA and PCR products along ± 1049 bp were detected. The results of the phylogenetic construction showed that PMSA1 was distributed into three allele groups, namely: K1 (8); MAD20 (1); and PMSA1_Sikka (11), dominated by the single PMSA1_Sikka allele. This study has found a different PMSA1 Sikka group with previously identified alleles, strengthened by in silico studies to produce a new candidate antigen epitope from Sikka isolates, immunogenic with a length of 17 aa and predicted to trigger an antibody response.