PCR Sequencing Protocols

Abstract

Preparation and Analysis of DNA Sequencing Gels Purification of PCR Products from Agarose Gels for Direct Sequencing Enzymatic Fluorescence and Biotin Labeling of Primers for PCR Sequencing Direct Sequencing of Double-Stranded PCR Products with the Sequencase Kit and [a-35S] dATP Direct Sequencing by Thermal Asymmetric PCR Rapid Sequencing of cDNA Clones: Direct Sequencing Using Sequential Linear/Asymmetric PCR Direct Sequencing of PCR Products Using Chemiluminescent Detection Direct DNA Sequencing of PCR Products Using Magnetic Beads Affinity Capture and Solid-Phase Sequencing Biotinylated PCR Products Analysis of Nucleotide Sequence Variations by Solid-Phase Minisequencing. Nonradioactive PCR Sequencing Using Digoxygenin Silver SequencingTM: Nonradioactive Cycle Sequencing of dsDNA Direct Sequencing of PCR Products with DNA-Binding Proteins PCR Sequencing with the Aid of Detergents Direct Sequencing with Highly Degenerate and Inosine-Containing Primers Determination of Unknown Genomic Sequences Without Cloning DNA Sequencing by the Chemical Method Direct PCR Sequencing with Denaturants (Formamide) Efficient PCR Production of Single-Stranded DNA Sequencing Templates Preparation and Direct Automated Cycle Sequencing of PCR Products Solid-Phase Automated Sequencing of PCR-Amplified Genomic DNA Cloning PCR Products for Sequencing in M13 Vectors Sequencing PCR Products Cloned into M13 Vectors Genome Amplification with Transcript Sequencing (GAWTS) DNA Rescue by Vectorette Method Sequencing (dAidT) of Cloned Mixed PCR Products from Microbial Populations Index