PENGARUH WAKTU ULTRASONIKASI TERHADAP KARAKTERISTIK FISIKA NANOPARTIKEL KITOSAN EKSTRAK ETANOL DAUN SUJI (Pleomele angustifolia) DAN UJI STABILITAS FISIKA MENGGUNAKAN METODE CYCLING TEST

Abstract

ABSTRACT
 The nanoparticle system encapsulates and protects flavonoids of suji leaves from photolysis and
 oxidation instability. Chitosan is a biocompatible, biodegradable and non-toxic nanoparticles
 forming polymer. Ultrasonication is a materials mixing technique under high vibration energy.
 The ultrasonication time affects the particle size. The aim of this study was to determine the effect
 of the ultrasonication time on physical characteristics of chitosan nanoparticles of suji (Pleomele
 angustifolia) leaf ethanol extract and its physical stability after cycling test. Ethanol extract of suji
 leaves (EEDS) was obtained by maceration using 70% ethanol. Chitosan EEDS nanoparticles were
 made under ionic gelation method using an ultrasonication time of 3 minutes (FI), 6 minutes (FII),
 and 9 minutes (FIII). The physical characterization of nanoparticles includes particle size,
 polydispersity index, and zeta potential. Chitosan EEDS nanoparticles with the best physical
 characteristics (FIII) were tested for stability using cycling test method. The data obtained were analyzed using the Anova statistical method with a 95% confidence level. The size of EEDS
 chitosan nanoparticles (nm) was significantly different in all formulas, FI (374.47), FII (288.43),
 and FIII (233.37). The polydispersity index of FI (0.38) and FIII (0.65) were significantly different,
 while FII (0.41) was not significantly different. The zeta potential (mV) FI (51.70), FII (46.10), and
 FIII (48.60) were not significantly different in all formulas. The physical characteristics of
 Formula III after Cycling Test showed a particle size of 455.0 nm, a polydispersity index of 0.174,
 and a zeta potential of 20.1 mV.
 Keywords: ultrasonication, nanoparticles, chitosan, cycling test, suji leaf (Pleomele angustifolia)