Pengaruh Suplemen Organik Terhadap Regenerasi Tunas Pisang Barangan Musa acuminata Colla. Secara In Vitro

Mustika Tuwo,Andi Ilham Latunra,Baharuddin Baharuddin,Tutik Kuswinanti,A Masniawati

doi:10.24843/metamorfosa.2021.v08.i01.p13

Abstract

The research purpose to know ability of natural supplements in supporting the regeneration of banana cv. Barangan Musa acuminata Colla. Explants were cultured on MS basal medium (control) with natural supplements: coconut water (CW) 5; 10; 15 and 20%, green bean sprout extract (GBSE) and sweet corn extract (SCE) at 50; 100; 150 and 200 g/l. After 4 weeks of cultivation, maximum multiplication shoots (7.11 ± 0.19), shoot length (2.00 ± 0.22), and number of leaves (4.00 ± 0.38) was obtained in MS + 200 g/l bean sprout extract. The addition of natural supplements to the medium showed better results on all parameters of observation than control. This results indicates the potential of natural supplements for supporting of Barangan Musa acuminata Colla. propagation in vitro culture.

Highlights

Bananas are large perennial herb (Musa spp.) belong to the monocotyledonous family Musaceae
Data analysis show that the treatment of bean sprout extract starting from concentrations of 100 to 200 g/L significantly influences shoots multiplication shoots in 21 day after incubation (DAI)
It is insignificantly different with the treatment of Murashige and Skoog (MS) + coconut water 15% (5.11 ± 0.51) and MS + 200 g/L sweet corn extract (6.00 ± 0.58)

Summary

Introduction

Bananas are large perennial herb (Musa spp.) belong to the monocotyledonous family Musaceae. Banana is an important and widely grown fruit crop in the tropical and subtropical regions of the world, both as a staple food as well as a major export commodity (Darvari et al, 2010; Rahman et al, 2013; Qamar et al, 2015). Banana is one of the horticultural crops that is important for improving the nutrition of the community. As a source of vitamins, minerals and carbohydrates, banana are digested (Hapsari and Lestari, 2016). Have a high commercial value and opportunity to be developed. It has excellent nutrient content and rich in minerals such as potassium, magnesium, phosphorus, iron, and calcium, contains vitamin C, B complex, B6 and serotonin that are active as neurotransmitters in brain function (Sunyoto, 2011)

Objectives

Methods

Results

Conclusion