Abstract
The use of pesticides in foods can be bad for human health, so the danger of pesticide use in food can damage cell metabolism or permeability of the cell membrane. This study aims to analyze the effect of Spirulina extract on the potential difference of egg cell membrane of albumin and vitellin chickens against anti channel blocker on diazinon. The contaminant used in this research is diazinon 600 EC. How to overcome the contamination of livestock products that can be harmful to health one of them using antioxidants. Thus, the administration of Spirulina extract can affect the potential response of egg cell membranes ( Gallus Gallus domesticus ) in the polluted food of diazinon. Potential rabbit egg membrane can be measured by using microelectrode associated with PicoScope 5000 series , then the data appears will be displayed through the PC. The results of this measurement showed that along with the increase of diazinon solution concentration, the potential value of egg cell membrane produced decreased. While the influence of Spirulina extracts in the egg (Gallus gallus domesticus) membrane that has been contaminated diazinon showed the potential value of membrane bigger/higher. This is because in the extract Spirulina has phenolic and flavonoid content that has the potential as an antioxidant that can be useful to reduce the level of free radical reactivity caused by diazinon.
Highlights
The use of pesticides in foods can be bad for human health, so the danger of pesticide use in food can damage cell metabolism or permeability of the cell membrane
This study aims to analyze the effect of Spirulina extract on the potential difference of egg cell membrane of albumin and vitellin chickens against anti channel blocker on diazinon
Nilai potensial membran sel telur ayam ras (Gallus domesticus) terjadi peningkatan seiring dengan pertambahan konsentrasi larutan diazinon, dan pada konsentrasi 0,7 ppm potensial membran sel semakin menuju ke negatif
Summary
1) Program Magister Ilmu Fisika, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Brawijaya 2) Jurusan Fisika, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Brawijaya. Penelitian ini bertujuan untuk menganalisa pengaruh pemberian ekstrak Spirulina terhadap beda potensial membran sel telur ayam albumin dan vitelin terhadap anti channel blocker pada diazinon. Pada pemberian ekstrak Spirulina dapat berpengaruh terhadap respon potensial membran sel telur ayam ras (Gallus gallus domesticus) dalam hasil pangan yang tercemar diazinon. Potensial membran sel telur ayam ras dapat di ukur dengan menggunakan mikroelektroda yang dihubungkan dengan PicoScope 5000 series, kemudian data yang muncul akan ditampilkan melalui PC. Hasil pengukuran ini menunjukkan bahwa seiring meningkatnya konsentrasi larutan diazinon maka nilai potensial membran sel telur ayam ras yang dihasilkan mengalami penurunan. Hal ini dikarenakan dalam ekstrak Spirulina memiliki kandungan fenolik dan flavonoid yang berpotensi sebagai antioksidan sehingga dapat bermanfaat untuk mengurangi tingkat kereaktifan radikal bebas yang ditimbulkan oleh diazinon. Kata Kunci: Potensial membran, Diazinon 600 EC, Spirulina, Mikroelektroda, dan PicoScope series 5000
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