Pengaruh Kinetin dan BAP terhadap Pertumbuhan dan Perkembangan Embrio Somatik Tanaman Sagu (Metroxylon sagu Rottb.)

Abstract

<p>Effect of Kinetin and BAP to Growth and Development of<br />Somatic Embryos of Sago Palm (Metroxylon sagu Rottb.).<br />Imron Riyadi. Somatic embryos induction in sago palm<br />(Metroxylon sagu Rottb.) have succesfully developed. Kinds<br />and concentration of plant growth regulators (PGR’s)<br />influence to growth and development of somatic embryos.<br />The research was conducted to determine the optimal concentration<br />of BAP and kinetin for proliferation, maturation<br />and germination of sago palm somatic embryos. Cotyledonstage<br />of somatic embryo derived from shoot tip cultures<br />cultured on Modified Murashige-Skoog (MMS) with halfstrength<br />macro-salts and added with 30 g/l sucrose, 2 g/l<br />gelrite, 1 g/l activated charcoal. pH of media was adjusted at<br />5.6 before sterilized. The media were supplemented with<br />0.1-2.0 mg/l BAP and 0.1-2.0 mg/l kinetin in combination with<br />0.01 mg/l ABA each for supporting growth and development.<br />The cultures was incubated at 26+1oC under a 12-h<br />photoperiod with lighting providing an intensity 20 μmoles<br />photons/m2/second for 11 weeks with replication 10 times.<br />The results showed that the highest of somatic embryo<br />proliferation was achieved in a culture medium with BAP at<br />0.5 mg/l + 0.01 mg/l ABA with an expression rate of 94%, the<br />best maturation at 1.0 mg/l kinetin + 0.01 mg/l ABA with an<br />expression rate of 93.5% and the most germination at 2.0<br />mg/l kinetin + 0.01 mg/l ABA with an expression rate of<br />100%. Transfer of these germinants to gelled media without<br />PGR’s led to the development of normal plantlets.</p>