Pengaruh Hormon Asam Indol Asetat yang Dihasilkan Azospirillum sp. terhadap Perkembangan Akar Padi

Abstract

<p>Free-living bacteria of the<br />genus Azospirillum live in close association with rice roots.<br />This bacteria produced indole acetic acid (IAA), a plant<br />growth hormon, to the environment. IAA was isolated from<br />cultures of Azospirillum strains and investigated for its effect<br />on root development and plant height of rice variety IR64 in<br />vitro. Rice cultures of variety IR64 were grown in vitro and<br />inoculated with cultures of Azospirilllum. Production of IAA<br />by the bacterium during its growth period in rice culture medium<br />containing different levels of nitrogen was observed.<br />Results of the experiment showed that strains Azospirillum<br />Az15 and Az44 had a high ability to produce IAA, i.e., 57.93<br />μg/ml at 12 days after incubation (DAI) and 40.42 μg/ml at 7<br />DAI, respectively. The IAA production pattern of Azospirillum<br />Az15 and Az44 in the liquid medium were fluctuative<br />until the end of the incubation period, while that of the strain<br />Az7 was linier. Strain Az7 gave a better effect on the root<br />development and plant height than strains Az15 and Az44.<br />Treatment combination of strain Az7 and 100% nitrogen gave<br />highest root development. High level of nitrogen increased<br />IAA content in the uninoculated culture, while low IAA<br />content on the inoculated one. Inoculation the culture with<br />strain Az7 together with 50% nitrogen application resulted in<br />the IAA content, root dry weight, root length, fiber root number,<br />and plant height as high as those on cultures containing<br />100% nitrogen (1 mM NH4NO3) without inoculation. Inoculation<br />of rice culture with Azospirillum is expected to reduce<br />nitrogen application on rice IR64 by the IAA production as<br />indicated by significant changes in the root growth and<br />development. A higher concentrations of IAA tend to give<br />better effects on the root growth and development of rice<br />IR64.</p>