Abstract
Costus speciosus has been reported to have biological activity among antidiabetic, antihyperlipidemia, antioxidants. One of the chemical constituents that is responsible for some of the biological activity is diosgenin. This study aimed to determine diosgenin content of Costus speciosus water extract (CS). Water extract of CS was obtained with infundation method. The extract was dried using freeze dryer. The assay of diosgenin was performed by HPLC method. HPLC system used: LiChrospher® 100 RP-18 endcapped (5 µm) column length 25cm, colimn id 4 mm as stationary phase, acetonitrile-water (9: 1 v/v) as mobile phase, flow rate 2mL/min and UV detection at 205 nm. HPLC method developed was linear in the range 20-200 μg mL with R = 0.9999 ; slope = 6423.7; and intercept = -4280.5; LOD = 2.14 ug/mL and LOQ = 6.50 mg/mL; and recovery 100.63 %. Diosgenin levels in CS was 0.279 %. The developed HPLC method is relatively simple, rapid, sensitive, and accurate to determine the content of diosgenin in water extracts of Costus speciosus.
Highlights
Costus speciosus has been reported to have biological activity among antidiabetic, antihyperlipidemia, antioxidants
Water extract of Costus speciosus water extract (CS) was obtained with infundation method
The assay of diosgenin was performed by HPLC method
Summary
Tanin, dan fenolik (Devi &Urooj, 2010) Enam senyawa yang diisolasi dari rimpang C. speciosus dan dijelaskan sebagai diosgenin(1), prosapogenin B dioscin(2), diosgenone(3), cycloartanol(4), 25-encycloartenol(5) dan asam octacosanoic(6) (Qiao et al.., 2002). Diosgenin (Gambar 2) adalah konstituen bioaktif yang sangat penting dalam herba pacing. Ada kebutuhan mendesak untuk mengembangkan metode sederhana untuk estimasi senyawa ini menggunakan instrumen canggih seperti kromatografi cair kinerja tinggi (HPLC). Beberapa metode analisis telah dikembangkan untuk analisis diosgenin hingga saat ini, antara lain: spektrofotometri HPLC, HPTLC, dan TLC (Chapagain and Wiesman, 2005; Amir et al, 2010, Abdelkarim et al, 2015). Baik metode spektrofotometri maupun metode HPTLC relatif kompleks karena sedikitnya kromofor yang terdapat pada struktur diosgenin, sehingga harus dilakukan derivatisasi. Pada penelitian ini dilakukan pengembangkan metode HPLC untuk menetapkan kadar diosgenin dalam ekstrak herba pacing agar diperoleh metode yang sensitif, akurat dan cepat. Bahan dan Alat Herba pacing (Costus speciosus) akuades (diperoleh dari distributor lokal), asetonitril (HPLC grade, E Merck), metanol pa.
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