Abstract
Avian influenza is a viral disease in poultry caused by avian influenza virus (AIV) subtype H5N1 with varying clinical signs are often similar to the clinical signs of other viral infections, such as Newcastle disease virus (NDV). The mechanism of disease pathogenesis to express clinical signs tightly correlated to the determination of the target organ seen from AIV H5N1 antigens distribution in organs, such as respiratory tract, brain and gastrointestinal tract. Immunopathological immunohistochemistry streptavidin-biotin (IHC SB) is a method for sensitive and accurate in detecting antigens of AIV on the tissues. In the present study, it was determined whether in laying hens with clinical signs of torticollis and curled toe paralysis, and pathologic anatomic lesions in the form of petechial and foci necrotic hemorrhages tested with immunopathological IHC SB is positive AIV H5N1 infection. IHC SB study results showed that the AIV H5N1 antigen were found in tissues of the lung, brain, duodenum and proventriculus. Based on these results, we can conclude that the IHC SB is a method that is highly sensitive and accurate to detect H5N1 antigens and its distribution in the host.
Highlights
Penyakit flu burung atau avian influenza (AI) merupakan penyakit viral yang disebabkan oleh virus flu burung atau virus avian influenza (VAI) subtipe H5N1, yaitu influenza tipe A yang termasuk dalam famili Orthomyxoviridae (OIE, 2015) yang pada unggas bersifat akut dengan tingkat morbiditas dan mortalitas tinggi
Avian influenza is a viral disease in poultry caused by avian influenza virus (AIV) subtype H5N1 with varying clinical signs are often similar to the clinical signs of other viral infections, such as Newcastle disease virus (NDV)
The mechanism of disease pathogenesis to express clinical signs tightly correlated to the determination of the target organ seen from AIV H5N1 antigens distribution in organs, such as respiratory tract, brain and gastrointestinal tract
Summary
Penyakit flu burung atau avian influenza (AI) merupakan penyakit viral yang disebabkan oleh virus flu burung atau virus avian influenza (VAI) subtipe H5N1, yaitu influenza tipe A yang termasuk dalam famili Orthomyxoviridae (OIE, 2015) yang pada unggas bersifat akut dengan tingkat morbiditas dan mortalitas tinggi. Mekanisme timbulnya torticollis dan curled toe paralysis akibat penempelan VAI pada reseptor sel inang pada unggas. Wabah AI dengan gejala klinis torticolis dan curled toe paralysis, serta lesi patologi anatomi petechial hemorrhages dan foci necrotic hemorrhages paru-paru terjadi di Indonesia pada tahun 2013. Penelitian ini ditujukan untuk menentukan ada atau tidaknya antigen (VAI) pada ayam petelur dengan gejala klinis torticollis dan curled toe paralysis, dan lesi patologi anatomi petechial hemorrhages dan foci necrotic hemorrhages pada paru-paru, saluran pencernaan, dan otak dengan pendekatan uji imunopatologis IHK SB. Diharapkan, uji IHK SB dapat dimanfaatkan dalam penentuan organ target VAI yang berguna dalam pemahaman patogenesis untuk menjelaskan mekanisme masuknya VAI ke Desember 2017 Vol 18 No 4 : 487-495 sistem saraf pusat sehingga berpengaruh terhadap timbulnya manifestasi gejala klinis neurotropism berupa torticollis dan curled toe paralysis pada ayam petelur. Selain itu penentuan organ target juga bermanfaat untuk membantu peneguhan diagnosis terkait dengan topografi anatomi distribusi VAI, sehingga dapat dibedakan antara infeksi flu burung dengan infeksi virus lain yang menimbulkan gejala klinis serupa, misalnya Newcastle disease virus (NDV)
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