Year-end Sale % OFF 
Abstract
<p>ABSTRACT</p><p>Salinity (salt stress) is one of the different causes of decline in crop yields and productivity of sugarcane. One strategy for optimizing the production of sugarcane on saline land required tolerant varieties of salinity. This research was conducted to determine NaCl concentration that caused more than &gt; 90% callus death on sugarcane (S officinarum) tissue culture and to investigate the protein profile of sugarcane callus BL and PS-862 varieties on saline (NaCl) stress condition. Sugarcane BL and PS-862 varieties were obtained from tissue culture. and those callus were sub-cultured in salinity media with composition of MS (Murashige-Skoog) as base medium and NaCl in various concentration (0%; 0.25%; 0.5%; 0.75%; and 1%). The results showed condition of NaCl addition on media by 1% concentration cause alteration on morphology of callus that 75% part of whole tissues become dark brown coloured that indicating the tissues death. At four weeks after sub-culture, the calli on both varieties obtain score 4. Lethal percentage of both varieties BL and PS-862 in 1% NaCl was 91,67%. This result were supported by protein profile analysis. that a thinner protein band in 1% NaCl treatment compared with control. This is indicating that in salinity stress causing the decrement of protein expression.</p><p> </p>
Highlights
Saat ini terjadi ketidak seimbangan antara produksi dan konsumsi gula
The results showed condition of NaCl addition on media by 1% concentration cause alteration on morphology of callus that 75% part of whole tissues become dark brown coloured that indicating the tissues death
Effect of Sodium Chloride Stress on the Plasma Membran ATPase of Barley Roots: Probable Cause for Decrease in ATPase Activity
Summary
Penelitian dilaksanakan mulai bulan Januari sampai dengan April 2013, di Laboratorium Kultur Jaringan Balai Penelitian Tanaman Pemanis dan Serat, Malang. Tahapan dalam kegiatan ini adalah sebagai berikut: Induksi kalus Eksplan tanaman tebu yang digunakan adalah daun tebu yang masih menggulung yang diinokulasikan pada media induksi kalus yaitu media MS (Murashige-Skoog) dengan tambahan ZPT 2.4-diklorofenoksiasetat (24-D) 3 ppm. Seleksi kalus pada media salin Kalus tebu dengan berat 0.1 g disub-kulturkan pada media seleksi yaitu media dasar MS yang ditambah NaCl dengan konsentrasi 0%; 0.25%; 0.5%; 0.75%; dan 1% selama 4 minggu. Pada 1 minggu setelah sub-kultur, dilakukan uji profil protein dengan metode SDS-PAGE terhadap kalus yang memiliki tingkat kematian >90%. Data yang diperoleh dilakukan analisis deskriptif untuk mengetahui respon masingmasing varietas terhadap konsentrasi NaCl. Skoring Kalus Kondisi morfologi kalus berbeda-beda setelah ke media salin (MS + NaCl), untuk mengamati kerusakan kalus akibat NaCl, dilakukan scoring dengan nilai 0-4, sebagai berikut: Skor 0 : kalus sehat. Gambar 1 Kondisi morfologi kalus tebu (Saccharum officinarum) varietas BL berdasarkan nilai skoring (Dokumentasi Pribadi. 2013)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
