Enhancing salinity stress tolerance in corn salad (Valerianella locusta L.) through melatonin or salicylic acid-functionalized chitosan seed priming: A smart delivery approach

Abstract

Soil salinity represents a significant environmental stressor that impairs crop production and yield. A wide range of treatments have been used to reduce the effects of salinity in plants. Among the treatments used, functionalized nanoparticles (NPs) have shown great results. In light of recently demonstrated beneficial effects of chitosan-melatonin nanoparticles (CTS-Mel NPs) and chitosan-salicylic acid nanoparticles (CTS-SA NPs) in mitigating the deleterious consequences of major stress factors and boosting secondary metabolite biosynthesis, the current study aimed to investigate their potential as seed priming coatings to enhance plant performance under both control and salinity stress conditions. For this purpose, CTS (0.1% w/v), Mel (50 µM), SA (0.5 mM), CTS-Mel NPs and CTS-SA NPs were applied as seed priming agents on corn salad (Valerianella locusta) seeds, and subsequently key morphophysiological and biochemical properties were assayed under salinity conditions (0, 30 and 60 mM NaCl). Accordingly, salinity stress caused significant reduction in fresh and dry weights (FW and DW) of leaves, chl a, total chl, SPAD and enhancement in content of proline, phenolics, MDA, as well as protein content, and activities of SOD and CAT antioxidant enzymes. Concerning the phenolic compounds analyzed, salinity stress did not affect the dominant phenolic constituents with the exception of naringine. Regarding the protective effects of the various priming treatments, the adverse effects of salinity stress were ameliorated with the application of most of the applied treatments, and with CTS-Mel NPs in particular, by enhancing biomass, pigments, total phenols, protein, SOD and CAT antioxidant enzymatic activities, as well as the content of some dominant constituents of phenolic profile. CTS-Mel NPs enhanced chlorogenic acid, naringine, o-coumaric acid and catechin hydrate under both control and salinity conditions. Overall, CTS-Mel NPs outperformed CTS-SA NPs as a seed priming coating and could potentially be widely introduced as an innovative, sustainable approach to mitigate the effects of salinity and other abiotic stress conditions in crop plants.