Abstract
Extended Abstract Curcumin is a polyphenol derived from the Curcuma Longa plant. Curcumin exhibits a variety of therapeutic properties, including antioxidant, analgesic, anti-inflammatory, anticancer and antiseptic activities [1]. Curcumin acts as photosensitizer in photodynamic therapy (PDT), which is an alternative way to kill pathogenic microorganisms [2]. The use of gold nanoparticles (AuNPs) as the vehicle for curcumin delivery increases photodynamic ability [3]. Therefore, the present study aim is to investigate the effect of the photodynamic action of LED-activated curcumin gold nanoparticles, on cell viability of Staphylococcus aureus, Staphylococcus aureus (Sa), Staphylococcus epidermidis (Se), Pseudomonas aeruginosa (Pa), Escherichia coli (Ec), Citrobacter freundii (Cf) and Klebsiella pneumoniae (Kp). To prepare Curcumin gold nanoparticles (Curc:AuNps) solutions, 3.2 mg of HAuCl4 was mixed with 1.5 mg Curcumin and Polyethylene glycol (PEG) in Mili-Q water. This solution was exposed to light from a mercury metal halide lamp. The resulting solutions were characterized by UV-Vis and transmission electron microscopy (TEM). The in vitro antibacterial activity of Curc:AuNps was evaluated against Sa, Se, Pa, Ec, Cf and Kp using the minimum inhibitory concentration (MIC) test. The effects of exposure to yellow LED light with ~38 J/cm fluence and variations in the Curc:AuNPs concentrations (50, 75 and 100% dilutions) were studied. The presence of a surface plasmon resonance band at ~520 nm indicated the formation of spherical gold nanoparticles. In the presence of PEG, the solution color changed some minutes after the mixing of reagents, and solution size homogeneity was improved with the mercury metal halide lamp illumination. TEM analyses showed ~17±2 nm nanoparticles. The results indicate that PDT with curcumin gold nanoparticles strongly inhibits the development of Gram positive and Gram negative bacteria. This study revealed antibacterial probability of inhibition >80% with 32 mM of Curc:AuNPs and light against Sa, Se, Cf and Kp strains after 18 hours of incubation. NOVA/Tukey's tests were conducted to compare groups curcumin/Curc:AuNPs, with and without photodynamic excitation. This work was supported by the “Fundacao de Amparo a Pesquisa do Estado de Sao Paulo” (FAPESP), Grant number 2014/06960-9.
Highlights
Curcumin, is a polyphenol that accumulates in the rhizome of Curcuma longa, can reduce the risk of heart disease, high blood pressure and cognitive decline[1,2,3,4,5,6,7,8]
Applying broth microdilution testing to a number of bacterial strains provides an estimate of the concentration that inhibits bacterial isolates and can indicate shifts in the susceptibility of bacterial populations to gold nanoparticles
The data from the experiments performed in triplicate are shown in Figures 3 referring to the incubation periods of 18 hours, without and with photodynamic therapy (PDT), respectively
Summary
Curcumin (diferuloylmethane), is a polyphenol that accumulates in the rhizome of Curcuma longa, can reduce the risk of heart disease, high blood pressure and cognitive decline[1,2,3,4,5,6,7,8]. Due to its capabilities to regulated important transcription factors, protein kinases, cytokines, adhesion molecules and redox status, curcumin can serve as an anti-inflammatory, antioxidant, anti-angiogenic, antimicrobial and antineoplasic agent[9]. Curcumin has been shown to inhibit cellular proteins FtsZ10 and sortase A11, thereby interrupting cytokinesis and cellular adhesion, and interfere with biofilm formation[12]. Though curcumin exhibits broad antimicrobial activity, some data suggest that Gram-positive species are more susceptible than Gram-negatives[1, 9]. Curcumin described as prebiotic, which have beneficial effects on human health
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