Peculiarities of vitamin D receptor gene polymorphism and its connection with indicators of metabolic status in children with obesity

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Objective — to identify peculiarities of vitamin D receptor gene polymorphism and its connection to indicators of metabolic status in children with obesity and normal body mass.Materials and methods. We have examined 39 children (19 boys and 20 girls) with obesity (body mass index (BMI) (30.6 ± 2.06) kg/m2, age (13.23 ± 2.27) years)).We have determined the main indicators of lipid and phosphorus-calcium metabolism, uric acid, glycated hemoglobin in biochemical analysis of blood. Hormonal state: insulin, leptin, adiponectin, cortisol, thyroid stimulating hormone, free thyroxine, vitamin D.We have conducted genotyping of vitamin D receptor gene by ApaI (A/a) (rs7975232), TaqI (T/t) (rs731236), BsmI (B/b) (rs1544410), FokI (F/f) (rs2228570) polymorphic alleles.Results and discussion. We have identified statistically significant differences by frequency of genotypes and ApaI (A/a) (rs7975232), TaqI (T/t) (rs731236), BsmI (B/b) (rs1544410) VDR alleles between boys with obesity and normal body mass.There have been no identified statistically significant differences by frequency of genotypes and TaqI (T/t) (rs731236), BsmI (B/b) (rs1544410), FokI (F/f) (rs2228570) VDR alleles in girls with obesity.In indicators of biochemical analysis of blood of patients with obesity by ApaI (A/a) (rs7975232) polymorphic locus we have identified statistically significant increase of (р(Аа) = 0.03; р(аа) = 0.006) triglyceride, (р(АА) = 0.004; р(аа) = 0.05) uric acid and (р(Аа) = 0.02) alkaline phosphatase level as to the (р(Аа) = 0.03; р(аа) = 0.006) control group.In patients with AA and Аа genotypes there has been determined a statistically significant increase of insulin indicators in blood serum as to the (р(АА) = 0.002; р(Аа) = 0.002) control group. We have identified a statistically significant difference by HOMA­IR index in children with obesity as to the (р(АА) = 0.007; р(Аа) = 0.03) control group. There has been a noted statistically significant difference by leptin level in children with obesity as to the (р(аа) = 0.007 control group with aa genotype.Conclusions. We have identified a statistically significant increase of triglyceride, HOMA­IR index, leptin and uric acid level in children with obesity by ApaI (A/a) (rs7975232) polymorphic locus as to the control group.