PECULIARITIES OF THE LACRIMAL FLUID CYTOKIN PROFILE IN PATIENTS WITH BACTERIAL KERATITITIS OF THE II DEGREE SEVERETY AND DIABETES MELLITUS

Abstract

Summary. The aim was to identify the features of the lacrimal fluid cytokine profile in patients with bacterial keratitis of the II degree of severity and type 1 DM in the dynamics of observation.
 Materials and methods. The lacrimal fluid cytokine profile was analyzed in 6 patients with type 1 DM and bacterial keratitis of the II degree of severity and 6 nondiabetic patients with bacterial keratitis of the II degree of severity on the 1st day, 10-14th day and 24th day of observation. Data from 14 healthy individuals of the appropriate age were used for comparison. II degree of severity of bacterial keratitis was determined according to our proposed scheme at a sum of points 15-21. All patients were treated with instillations of ofloxacin, antiseptics, antioxidants, reparents, artificial tears, mydriatics, systemic anti-inflammatory therapy. The concentration of interleukins (IL)-1β, IL-6 and IL-10 in the lacrimal fluid of the sick and the contralateral eye was determined by quantitative colorimetric enzyme-linked immunosorbent assay using ELISA kits.
 Results and discussion. In patients with bacterial keratitis of the II degree of severity, both with DM and nondiabetic, within 10-14 days of observation invented the expression of proinflammatory cytokines IL-1β and IL-6 in the lacrimal fluid of the sick eye and anti-inflammatory cytokine IL-10 - in lacrimal fluid of the contralateral eye (p<0.05). In DM patients, a longer expression of IL-1β and IL-6 was detected in the lacrimal fluid of the sick and the contralateral eye, which was observed on the 24th day of the study (p<0.05).
 Conclusions. There are features of the lacrimal fluid cytokine profile in DM patients with bacterial keratitis of the II severity in the dynamics of observation, namely the longer expression of proinflammatory cytokines IL-1β, IL-6 in the sick and the contralateral eye.