Abstract
Thirty-seven males with non-alcoholic staatohepatitis (NASH) (aged 39 - 62) were included in the study. Clinical, biochemical and instrumental studies were used to verify the diagnosis. The control group was comprised of thirty-three males of comparable age and without confirmed signs of liver diseases and other pathology of inner organs. Dielectrophoresis was applied to evaluate the structural-and-functional erythrocytic parameters. The patients with NASH erythrocytes tended to differ in their much lower amplitude deformation, polarizability, capacity of erythrocytic membranes, pace of translational motion of cells towards electrodes and much higher levels of overall indices of rigidity, viscosity, electroconductivity, destruction and aggregation as compared to the control group (p < 0.001 - 0.05). We discovered cases of correlation of erythrocytic parameters with biochemical findings reflecting inflammatory and cytolytic syndrome, cholestasis, protein-synthesizing liver function as well as markers of metabolic syndrome (BMI, changes in arterial blood pressure, the degree of disturbances in carbohydrate metabolism, atherogenic dyslipidemia) and microalbuminuria. Aggravation of microcirculatory disturbances leading to an increase of fibrogenes is induced by changes in the properties of erythrocytes under NASH suggests the administration of a number of therapeutic techniques which could improve the status of the parameters of red blood cells. This study aims to evaluate the peculiarities of electric and viscoelastic behavior of erythrocytes in patients with primary NASH in order to determine additional methods of treatment for this disease.
Highlights
Nonalcoholic steatohepatitis (NASH) is a heterogenic group of pathologic changes in the liver characterized by inflammatory infiltration against a background of fatty degeneration of hepatocytes in individuals who do not consume alcohol in hepatotoxic dosages
Studies of viscoelastic and electric erythrocytic parameters in patients with NASH demonstrated that red blood cells display a significantly lower amplitude of deformation
Erythrocytes in patients with NASH tended to significantly differ in their lower deformation amplitude induced by non-uniform alternating electric field (NUAEF), polarizability, capacity of membranes, pace of shift of cells to electrodes and in the significantly higher levels of summarized rigidity, viscosity, electroconductivity, indices of destruction and aggregation as compared to the control group
Summary
Nonalcoholic steatohepatitis (NASH) is a heterogenic group of pathologic changes in the liver characterized by inflammatory infiltration against a background of fatty degeneration of hepatocytes in individuals who do not consume alcohol in hepatotoxic dosages. The importance of early diagnosis and treatment of NASH is preconditioned by the following facts: an examination of large groups of patients with cryptogenic liver cirrhosis, including the evaluation of concomitant diseases and risk factors, led to the assumption that in 60% - 80% of cases, liver cirrhosis of unclear etiology is based on undiagnosed NASH [1]. In the USA, 2% - 6% of the total number of liver transplantations are known to be performed on NASH patients with decompensated liver cirrhosis [1]. Taking into account that erythrocytes are the most numerous blood cells and play an important role in maintaining homeostasis, changes in erythrocytic parameters in patients with metabolic syndrome are known to inevitably aggravate the course of NASH [1]. Therapeutic measures aimed to normalize such parameters of red blood cells as deformity, electroconductivity, capacity of membranes, etc. (along with treatment of manifestations of metabolic syndrome and removal of risk factors) that would obviously result in the minimization of manifestations of NASH
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Analytical Sciences, Methods and Instrumentation
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
