Peculiarities of cytokine and chemokine content in blood serum of children with psoriasis vulgaris

Abstract

Psoriasis is a chronic auto-inflammatory skin disease with a hereditary predisposition due to the activation of T-lymphocytes and the synthesis of pro-inflammatory cytokines. The pathogenesis involves populations of Th1 and Th17 lymphocytes that produce TNFa, IFNy, IL-17, IL-21 and IL-23. The aim of the study was to identify the features of the content of cytokines and chemokines in the blood serum of children with psoriasis. 88 children with psoriasis of varying severity were examined. The level of circulating cytokines was determined by the multiplex method on the Bio-plex 200 device using the MILLIPLEX MAP Human Th17 kit. In children with psoriasis, increased levels of IL-23, IL-22, IL-12p70, IL-27, IL-17E, IL-17F and TNFa were detected. The consistency of the functioning of the cytokine network in children with psoriasis was shown, which was expressed in the high strength of the connection between cytokines of the IL-17 family and cytokines of the IL-12 family, between IL-31 and IL-33, as well as in significant correlations between GM-CSF and cytokines IL-2, IL-5, IL-9, IL-10, IL-15 and TNFa. In children with psoriasis, the dependence of the level of IL-17A, TNFa, IL-2, IL-6, IL-9, CCL20/MIP3a with the severity of the disease according to PASI was revealed. The study confirmed the pathogenetic role of the cytokine network in the development and maintenance of an inflammatory response in children with psoriasis. The data obtained substantiate the need for targeted anti-cytokine therapy. The level of proinflammatory cytokines in children with psoriasis can be used as an additional laboratory criterion for the severity of the condition and monitoring the effectiveness of therapy.