Abstract
Wood particles of pine required longer treatments of acidic chlorite and dilute alkali to achieve similar proportions of cell separation as of poplar. Chemical extraction and enzymatic digestion studies indicated that rhamnogalacturonan-I (RG-I), mannan and xylan contribute to cell-cell adhesion in pine wood. Mannan associated with lignin in an acidic chlorite fraction and with xylan in a dilute alkali fraction. RG-I and xylan were extracted in both chlorite and dilute alkali in poplar. Scanning electron microscopy and a probe diffusion study using Fluorescence Recovery After Photobleaching showed that lignin, RG-I and mannan initiated cell separation at cell corners and dilute alkali extraction of xylan was required for complete cell separation in pine. Cell-cell separation increased enzymatic saccharification within 24 h by 2-fold in poplar and 3.4-fold in pine. Our results inform a strategy to reduce particle size in woody biomass and provide insights into different molecular bases of cell-cell adhesion. • Sequential extraction by acidic chlorite and dilute alkali of pine and poplar disrupted cell-cell adhesion. • After delignification, a combination of mannanase, xylanase, and RG lyase disrupted cell-cell adhesion. • Mannan, xylan, and RG-I determine cell-cell adhesion in pine and poplar by associating with different cell wall fractions. • Higher proportions of separated cells showed higher yields of enzymatic digestion.
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