PD36-01 HYPOGASTRIC AND PELVIC NERVE CRUSH INJURY IMPAIRS VAGINAL MICROVASCULAR PERFUSION IN A RAT MODEL

Abstract

You have accessJournal of UrologySexual Function/Dysfunction: Female (PD36)1 Apr 2020PD36-01 HYPOGASTRIC AND PELVIC NERVE CRUSH INJURY IMPAIRS VAGINAL MICROVASCULAR PERFUSION IN A RAT MODEL Samuel Sorkhi*, Min Chul Cho, Sung Yong Cho, Hong Chung, Christopher Cano Sanchez, Valmik Bhargava, and M. Raj Rajasekaran Samuel Sorkhi*Samuel Sorkhi* More articles by this author , Min Chul ChoMin Chul Cho More articles by this author , Sung Yong ChoSung Yong Cho More articles by this author , Hong ChungHong Chung More articles by this author , Christopher Cano SanchezChristopher Cano Sanchez More articles by this author , Valmik BhargavaValmik Bhargava More articles by this author , and M. Raj RajasekaranM. Raj Rajasekaran More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000000907.01AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Radical hysterectomy (RH), the most common surgical approach to treat early-stage cervical carcinoma results in damage to the autonomic nerves (AN) that supply female genital organs (pelvic plexus, hypogastric and pelvic nerves), as well as vaginal fibrosis leading to female sexual dysfunction. Even with nerve-sparing techniques, impaired neural control of vasocongestion during sexual arousal may arise due to AN damage that supply the vaginal wall and clitoris. Impact of AN injury (ANI) on vaginal and clitoral hemodynamics is unclear. The objectives of our study are to: (A) evaluate vaginal and clitoral blood perfusion (microvascular perfusion-MVP; using via laser speckle contrast imaging; LSCI) changes and (B) to evaluate the effect of transpelvic magnetic stimulation (TPMS) after ANI in a female rat model. METHODS: We used female rats and created AN crush injury (ANI; Fig A: schematic of rat pelvic neural innervation and AN crush and Fig B photomicrograph showing MPG) that simulates RH related nerve damage in women. The rats were anesthetized, pelvic organs were accessed via a lower-midline abdominal incision, and AN were crushed by applying a microsurgical hemostat for 3 x 30 second intervals. After recovery, the rats were further subjected to TPMS with simultaneous monitoring of vaginal microvascular perfusion (MVP) using laser speckle contrast imaging (LSCI; Fig C) by PeriCam PSI system that quantifies MVP in arbitrary perfusion units (AU). RESULTS: Representative MVP images from control and ANI rats are shown in Fig D. TPMS-induced MVP enhancement was lower (∼70-80%; Fig E) in ANI rats when compared to controls. CONCLUSIONS: ANI female rat model is a viable animal model to test RH related genital hemodynamic impairment and TPMS will facilitate genital MVP improvement and possibly prevent fibrosis. Source of Funding: VA Merit Award © 2020 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 203Issue Supplement 4April 2020Page: e723-e723 Advertisement Copyright & Permissions© 2020 by American Urological Association Education and Research, Inc.MetricsAuthor Information Samuel Sorkhi* More articles by this author Min Chul Cho More articles by this author Sung Yong Cho More articles by this author Hong Chung More articles by this author Christopher Cano Sanchez More articles by this author Valmik Bhargava More articles by this author M. Raj Rajasekaran More articles by this author Expand All Advertisement PDF downloadLoading ...