PD27-03 EARLY DETRUSOR CHEMODENERVATION AFTER SPINAL CORD INJURY CAN REDUCE BLADDER HYPERTROPHY AND FIBROSIS

Abstract

You have accessJournal of UrologyUrodynamics/Lower Urinary Tract Dysfunction/Female Pelvic Medicine: Basic Research & Pathophysiology (PD27)1 Sep 2021PD27-03 EARLY DETRUSOR CHEMODENERVATION AFTER SPINAL CORD INJURY CAN REDUCE BLADDER HYPERTROPHY AND FIBROSIS Zin Khiang, Juliana Bushnell, Jeffrey Hyde, and Claire Yang Zin KhiangZin Khiang More articles by this author , Juliana BushnellJuliana Bushnell More articles by this author , Jeffrey HydeJeffrey Hyde More articles by this author , and Claire YangClaire Yang More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000002020.03AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Following spinal cord injury (SCI), pathological reflexes develop, altering bladder and sphincter function, and resulting in detrusor changes within weeks after injury. Detrusor chemodenervation is known to ablate the pathological reflexes, but its effects on the development of detrusor changes is unknown. In a rodent model of SCI, we examine the effect of early detrusor chemodenervation. METHODS: Adult female Sprague Dawley rats (250–300g) were given a severe contusion SCI with 200-kDyne force with zero dwell time using an Infinite Horizon device following laminectomy, at spinal level T9 (N=8). A group of control rats did not undergo spinal injury (N=3) but underwent laminectomy only. The SCI rats immediately underwent laparotomy, and either saline (N=4) or botulinum toxin A (BoNT-A) 10U (N=4) was injected into the detrusor in 8 equal aliquots of 3 microliters per injection. The animals were sacrificed at 8 weeks post injury (wpi), and the bladders harvested and weighed. Tissue was stained using hematoxylin and eosin and Masson’s trichrome. RESULTS: SCI without bladder chemodenervation (SCI-saline injected) resulted in significantly heavier bladders compared to the bladders of control (spinal-intact) animals (241±25 mg vs. 137±30 mg; p<0.01). BoNT-A injected bladders of SCI rats (SCI-BoNT-A) were significantly lighter compared to saline injected bladders of SCI rats (183±42 mg vs. 241±25 mg; p<0.05). SCI-saline rats had thicker bladder walls due to detrusor hypertrophy and fibrosis compared to bladders from control animals (339±89 mm vs. 193±48 mm; p<0.0002). Bladder wall thickness in the SCI-BoNT-A rats was significantly less compared to SCI-saline rats (202±55 mm vs. 339±89 mm; p<0.0001) (Figure). CONCLUSIONS: Chemodenervation immediately following SCI significantly mitigates the development of pathologic detrusor histology by reducing the development of fibrosis and hypertrophy. Source of Funding: William E. Bradley Family Foundation © 2021 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 206Issue Supplement 3September 2021Page: e444-e444 Advertisement Copyright & Permissions© 2021 by American Urological Association Education and Research, Inc.MetricsAuthor Information Zin Khiang More articles by this author Juliana Bushnell More articles by this author Jeffrey Hyde More articles by this author Claire Yang More articles by this author Expand All Advertisement Loading ...