PD22-04 ORAL ADMINISTRATION OF NANO-SIZED SILICON PARTICLES REGULATES GENE EXPRESSION RELATED TO OXIDATIVE STRESS AND SUPPRESSES RAT RENAL ISCHEMIA-REPERFUSION INJURY

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You have accessJournal of UrologyTransplantation & Vascular Surgery: Renal Transplantation & Vascular Surgery I (PD22)1 Apr 2020PD22-04 ORAL ADMINISTRATION OF NANO-SIZED SILICON PARTICLES REGULATES GENE EXPRESSION RELATED TO OXIDATIVE STRESS AND SUPPRESSES RAT RENAL ISCHEMIA-REPERFUSION INJURY Masataka Kawamrua*, Ryoichi Imamura, Yuki Kobayashi, Ayumu Taniguchi, Shigeaki Nakazawa, Taigo Kato, Toyofumi Abe, Hikaru Kobayashi, and Norio Nonomura Masataka Kawamrua*Masataka Kawamrua* More articles by this author , Ryoichi ImamuraRyoichi Imamura More articles by this author , Yuki KobayashiYuki Kobayashi More articles by this author , Ayumu TaniguchiAyumu Taniguchi More articles by this author , Shigeaki NakazawaShigeaki Nakazawa More articles by this author , Taigo KatoTaigo Kato More articles by this author , Toyofumi AbeToyofumi Abe More articles by this author , Hikaru KobayashiHikaru Kobayashi More articles by this author , and Norio NonomuraNorio Nonomura More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000000872.04AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Organ ischemia reperfusion injury (IRI) is unavoidable in kidney transplantation and induces reactive oxygen species and causes organ damage. Although the effectiveness of hydrogen for IRI has been reported for a long time, oral ingestion of hydrogen water and inhalation of hydrogen gas are difficult to apply widely in terms of efficiency and general use. We succeeded in generating a large amount of hydrogen by crushing silicon to nano-sized particle and reacting with water. A silicone component agent was administered to a rat model of renal IRI to examine whether renal injury could be suppressed. METHODS: Six-week-old male SD rats were administered a silicon component feed containing 1% nano-sized silicon particles and IRI surgery was performed one week later. This group was made into a silicon component group (IRI + Si group). An IRI group that administers normal diet and performs IRI surgery and a sham group that performs only right nephrectomy were prepared, and each group was compared 72 hours after surgery. RESULTS: Serum creatinine levels and urine protein significantly decreased in the IRI + Si group,compared to the IRI group. Serum malondialdehyde and urinary 8-hydroxydeoxyguanosine, which are oxidative stress markers, were also significantly decreased in the IRI + Si group. Transcriptome analysis by microarray showed that in the IRI + Si group, the expression of genes related to immune response, cytokine production, and apoptotic signal was decreased in comparison with the IRI group. Furthermore, suppression of mRNA expression level of inflammatory cytokines by quantitative PCR and reduction of renal tubular epithelial cell apoptosis by immunohistochemical evaluation were obtained, suggesting anti-inflammatory and anti-apoptotic effects. CONCLUSIONS: Rat renal IRI was suppressed by oral administration of nano-sized silicon particles, which is a novel hydrogen administration method. Source of Funding: This research was supported by Grants-in-aid for Scientific Research (18K16697) and Center of Innovation Science and Technology based Radical Innovation and Entrepreneurship Program (COI STREAM). © 2020 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 203Issue Supplement 4April 2020Page: e460-e460 Advertisement Copyright & Permissions© 2020 by American Urological Association Education and Research, Inc.MetricsAuthor Information Masataka Kawamrua* More articles by this author Ryoichi Imamura More articles by this author Yuki Kobayashi More articles by this author Ayumu Taniguchi More articles by this author Shigeaki Nakazawa More articles by this author Taigo Kato More articles by this author Toyofumi Abe More articles by this author Hikaru Kobayashi More articles by this author Norio Nonomura More articles by this author Expand All Advertisement PDF downloadLoading ...