PD11-01 EXTRACELLULAR COLLAGENIC TYPE AND STRUCTURAL ORGANIZATION CHANGES IN PROSTATE CANCER AND BENIGN PROSTATIC HYPERPLASIA

Abstract

You have accessJournal of UrologyBenign Prostatic Hyperplasia: Basic Research & Pathophysiology (PD11)1 Sep 2021PD11-01 EXTRACELLULAR COLLAGENIC TYPE AND STRUCTURAL ORGANIZATION CHANGES IN PROSTATE CANCER AND BENIGN PROSTATIC HYPERPLASIA Paolo Vota, Fabio Grizzi, Pieve Emanuele, Matteo Zanoni, Mattia Nicola Sangalli, Nicola Frego, Pieve Emanuele, Cinzia Mazzieri, Giovanni Toia, Alberto Mandressi, Nicolò Buffi, Giovanni Lughezzani, Pieve Emanuele, Massimo Lazzeri, Giorgio Guazzoni, Pieve Emanuele, and Gian Luigi Taverna Paolo VotaPaolo Vota More articles by this author , Fabio GrizziFabio Grizzi More articles by this author , Pieve EmanuelePieve Emanuele More articles by this author , Matteo ZanoniMatteo Zanoni More articles by this author , Mattia Nicola SangalliMattia Nicola Sangalli More articles by this author , Nicola FregoNicola Frego More articles by this author , Pieve EmanuelePieve Emanuele More articles by this author , Cinzia MazzieriCinzia Mazzieri More articles by this author , Giovanni ToiaGiovanni Toia More articles by this author , Alberto MandressiAlberto Mandressi More articles by this author , Nicolò BuffiNicolò Buffi More articles by this author , Giovanni LughezzaniGiovanni Lughezzani More articles by this author , Pieve EmanuelePieve Emanuele More articles by this author , Massimo LazzeriMassimo Lazzeri More articles by this author , Giorgio GuazzoniGiorgio Guazzoni More articles by this author , Pieve EmanuelePieve Emanuele More articles by this author , and Gian Luigi TavernaGian Luigi Taverna More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000001986.01AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: It is now ascertained that stromal-epithelial interactions play a crucial and poorly understood role in carcinogenesis and prostate cancer progression. Tumor stroma is a complex and dynamic set of cells that includes a fibroblastic component often referred to as cancer-associated fibroblasts and a collagenic and non-collagenic extracellular reactive matrix. METHODS: In the present study we investigate the collagenic extracellular reactive matrix in a series of prostate cancer biopsy specimens and benign prostatic hyperplasia (BPH) following transurethral resection of the prostate (TURP). Particularly the study focused on the type of collagen composition and its spatial organization. Sixty prostate specimens were investigated. Fifty specimens were diagnosed as prostate carcinoma and 15 as benign prostate hyperplasia. The samples were fixed in 10% formaldehyde and paraffin-embedded. Two-micrometer thick sections were cut and stained with Sirius red staining to distinguish type I and III collagen using a polarized light microscopy. The ratios of collagen I/III were automatically evaluated using a computer-aided image analysis system. The spatial organization was evaluated on unstained tissue sections by combining a multi-photon microscopy and an open-source MATLAB software framework that includes two separate but linked packages "CurveAlign" and "CT-FIRE". RESULTS: By observing the stained sections with Picro-Sirius Red we found different conformations of the collagenic extracellular matrix. Collagen matrix is characterized by a set of highly irregular fragments with different size, size and roughness. In particular, the tumor microenvironment consists of thin collagen fibers while dense plaques have been observed in the microenvironment that characterizes BPH status. Additionally, we found that in BPH type III collagen is less represented if compared to the low and high-grade tumoral tissues. A statistically significant difference was identified between BPH and biopsies of patients with low-grade tumor and in whose fragment no neoplastic cells were observed (p <0.001). In addition, the alignment of collagen fibers is much more pronounced in biopsy of prostate cancer patients than in tissues of patients with BPH. Collagen type (type I versus type III) composition and its spatial organization i.e. alignment is different when evaluated in tumoral versus inflammatory state. CONCLUSIONS: Collagen type (type I versus type III) composition and its spatial organization i.e. alignment is different when evaluated in tumoral versus inflammatory state. Given the dynamical process of tissue matrix remodeling, our findings first demonstrated that stromal collagen alignment might provide additional, clinically relevant information about prostate cancer and underscores the importance of stroma-cancer interactions. Source of Funding: None © 2021 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 206Issue Supplement 3September 2021Page: e197-e197 Advertisement Copyright & Permissions© 2021 by American Urological Association Education and Research, Inc.MetricsAuthor Information Paolo Vota More articles by this author Fabio Grizzi More articles by this author Pieve Emanuele More articles by this author Matteo Zanoni More articles by this author Mattia Nicola Sangalli More articles by this author Nicola Frego More articles by this author Pieve Emanuele More articles by this author Cinzia Mazzieri More articles by this author Giovanni Toia More articles by this author Alberto Mandressi More articles by this author Nicolò Buffi More articles by this author Giovanni Lughezzani More articles by this author Pieve Emanuele More articles by this author Massimo Lazzeri More articles by this author Giorgio Guazzoni More articles by this author Pieve Emanuele More articles by this author Gian Luigi Taverna More articles by this author Expand All Advertisement Loading ...