MP64-17 EXTRACELLULAR COLLAGENIC TYPE AND STRUCTURAL ORGANIZATION CHANGES IN PROSTATE CANCER AND BENIGN PROSTATIC HYPERPLASIA

Abstract

You have accessJournal of UrologyProstate Cancer: Basic Research & Pathophysiology II1 Apr 2018MP64-17 EXTRACELLULAR COLLAGENIC TYPE AND STRUCTURAL ORGANIZATION CHANGES IN PROSTATE CANCER AND BENIGN PROSTATIC HYPERPLASIA Fabio Grizzi, Alberto Mandressi, Piergiuseppe Colombo, Sara Melegari, Matteo Justich, Giorgio Bozzini, Mauro Seveso, Oliviero De Francesco, Nicolò Buffi, Giovanni Lughezzani, Massimo Lazzeri, Rodolfo Hurle, Luisa Pasini, Alessio Benetti, Silvia Zandegiacomo, Roberto Peschechera, Paolo Casale, Giorgio F. Guazzoni, and Gianluigi Taverna Fabio GrizziFabio Grizzi More articles by this author , Alberto MandressiAlberto Mandressi More articles by this author , Piergiuseppe ColomboPiergiuseppe Colombo More articles by this author , Sara MelegariSara Melegari More articles by this author , Matteo JustichMatteo Justich More articles by this author , Giorgio BozziniGiorgio Bozzini More articles by this author , Mauro SevesoMauro Seveso More articles by this author , Oliviero De FrancescoOliviero De Francesco More articles by this author , Nicolò BuffiNicolò Buffi More articles by this author , Giovanni LughezzaniGiovanni Lughezzani More articles by this author , Massimo LazzeriMassimo Lazzeri More articles by this author , Rodolfo HurleRodolfo Hurle More articles by this author , Luisa PasiniLuisa Pasini More articles by this author , Alessio BenettiAlessio Benetti More articles by this author , Silvia ZandegiacomoSilvia Zandegiacomo More articles by this author , Roberto PeschecheraRoberto Peschechera More articles by this author , Paolo CasalePaolo Casale More articles by this author , Giorgio F. GuazzoniGiorgio F. Guazzoni More articles by this author , and Gianluigi TavernaGianluigi Taverna More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2018.02.2062AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES In the present study we investigate the collagenic extracellular reactive matrix in a series of prostate cancer biopsy specimens and benign prostatic hyperplasia (BPH) following transurethral resection of the prostate (TURP). Particularly the study focused on the type of collagen composition and its spatial organization. METHODS 60 prostate specimens were investigated, 50 prostate carcinoma and 15 BPH. The samples were fixed in 10% formaldehyde and paraffin-embedded. Two-micrometer thick sections were cut and stained with picric acid-sirius red staining to distinguish type I and III collagen using a polarized light microscopy. The ratios of collagen I/III were automatically evaluated using a computer-aided image analysis system. The spatial organization was evaluated on unstained tissue sections by combining a multi-photon microscopy and an open-source MATLAB software framework that includes two separate but linked packages ″CurveAlign″ and ″CT-FIRE″. All of the data were analyzed using Statistica software (StatSoft, Inc., Tulsa, OK, USA) and GraphPad Prism 5 (San Diego, California, USA). RESULTS By observing the stained sections with Picro-Sirius Red we found different conformations of the collagenic extracellular matrix. Collagen matrix is characterized by a set of highly irregular fragments with different size, size and roughness: tumor microenvironment consists of thin collagen fibers while dense plaques have been observed in the microenvironment that characterizes BPH . Additionally, we found that in BPH type III collagen is less represented if compared to the low and high-grade tumoral tissues. A statistically significant difference was identified between BPH and biopsies of patients with low-grade tumor and in whose fragment no neoplastic cells were observed (p<0.001). In addition, the alignment of collagen fibers is much more pronounced in biopsy of prostate cancer patients than in tissues of patients with BPH. CONCLUSIONS Collagen type (type I versus type III) composition and its spatial organization i.e. alignment is different when evaluated in tumoral versus inflammatory state. Given the dynamical process of tissue matrix remodeling, our findings first demonstrated that stromal collagen alignment might provide additional, clinically-relevant information about prostate cancer and underscores the importance of stroma-cancer interactions. © 2018FiguresReferencesRelatedDetails Volume 199Issue 4SApril 2018Page: e857 Advertisement Copyright & Permissions© 2018MetricsAuthor Information Fabio Grizzi More articles by this author Alberto Mandressi More articles by this author Piergiuseppe Colombo More articles by this author Sara Melegari More articles by this author Matteo Justich More articles by this author Giorgio Bozzini More articles by this author Mauro Seveso More articles by this author Oliviero De Francesco More articles by this author Nicolò Buffi More articles by this author Giovanni Lughezzani More articles by this author Massimo Lazzeri More articles by this author Rodolfo Hurle More articles by this author Luisa Pasini More articles by this author Alessio Benetti More articles by this author Silvia Zandegiacomo More articles by this author Roberto Peschechera More articles by this author Paolo Casale More articles by this author Giorgio F. Guazzoni More articles by this author Gianluigi Taverna More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...