Abstract
Background and Purpose:The Fusarium species are among the most important fungi in the medical, veterinary and agricultural fields. Materials and Methods:In the present study, 172 strains of these fungi have been analyzed. The high molecular weight DNAs were extracted from 23 reference strains as well as from 149 isolated Fusarium species. Using the designed nucleotide primers from rDNA of Fusarium species, PCR analysis was performed for the amplification of ITS regions. Afterwards, the location of the effective endonuclease enzymes has been evaluated within approximately 930 bp of rDNA sequence. Results:Through the selected enzymes including; HhaI, MspI, TaqI and FaqI, the mentioned Fusarium species have been divided into 33 groups. The first three enzymes were able to classify Fusarium species into 23 groups of which 19 groups included one member, one group included two members and three groups included three members of the Fusarium species. This study also revealed the possibility in the identification of F. semitectum, F. solani complex, F. pseudograminearum, F. nisikadoi, F. coeruleum and F. acuminatum species by one unique enzyme. In addition, our study indicated the ability of the differentiation of F. Compactum from F. equiseti.Conclusion:As Compared to previous studies with more endonuclease enzymes and with limited in identifications, the ITS-RFLP patterns reported here an attempted to evaluate most of the Fusarium species successfully.
Highlights
The Fusarium species can incite directly the diseases in plants, humans and domesticated animals
The polymerase chain reaction (PCR) technique is a sensitive, rapid and a reliable diagnosis method in species identification which will enable us to overcome the poor sporulation of the Fusarium spp. and its identification [5, 6]
The aim of the current study was to evaluate the utility of PCR-RFLP of the ITS region for discriminating the Fusarium species
Summary
The Fusarium species can incite directly the diseases in plants, humans and domesticated animals. Fusarium spp. produce secondary metabolites associated with plant diseases, as well as with cancer and other growth defects in humans and domestic animals [2,3,4]. The discrimination of Fusarium spp. has been done based on the morphological characteristics such as the shape and the size of the macroconidia, the presence/absence of microconidia and chlamydospores and the colony morphology. Conclusion: As Compared to previous studies with more endonuclease enzymes and with limited in identifications, the ITS-RFLP patterns reported here an attempted to evaluate most of the Fusarium species successfully.
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