Characterisation, pathogenicity and hydrolytic enzyme profiling of selected Fusarium species and their inhibition by novel coumarins.

Abstract

Three Fusarium species isolated locally were characterised by the amplification of their rDNA ITS region, host specificity, and hydrolytic enzyme production. The strains were identified as Fusarium pseudoanthophilum, which is being reported for the first time in South Africa, as well as F. foetens and F. fujikuroi. All the three strains were capable of infecting vegetables such as tomatoes, bell and cayenne peppers, belonging to the Solanaceae family. The Fusarium strains also showed significant production of cell wall degrading enzymes in vitro, such as amylase, cellulase, xylanase, and polygalacturonase, thus highlighting the possibilities of these enzymes as pathogenic factors. Subsequently, the strains were discovered to be susceptible to three halogenated coumarins. The most effective of the tested coumarins, 6-bromo3-2,2-dibromoacetyl-2H-chromen-2-one, showed MIC values of 0.125, 0.0625 and 0.125mg/ml against F. foetens, F. pseudoanthophilum and F. fujikuroi, respectively. The antifungal potentials of the halogenated coumarins were confirmed in silico through PASS analysis, toxicity prediction and docking studies. Findings from this study demonstrate the use of these coumarins as potential control agents against the Fusarium species and other pathogenic fungi in general.