PCR-RFLP Characterization and Antifungal Susceptibility of Isolated Yeast Species from Different Sources in Egypt

Abstract

Yeasts are thought to contribute significantly to microbiological contamination. In this study, the aim was to examine the occurrence of yeast from different sources (pasturized kareish cheese, chicken meat, wild birds and woman vulvaginal swabs) and assess the antifungal susceptibility of the isolated yeast strains. Two hundred random samples (50 of each) Pasteurized kariesh cheese samples were collected from retail shops, mouth swab of pigeon farmhouses which were complain of loss of appetite and white cheesy exudate inside mouth, fecal droppings of Doves collected from roof of Hospital, General Gardens at Menufia, governorate, Egypt and vaginal swabs from women suffering of vulvovaginitis around Menufia governorate, Egypt hospitals. All samples were collected at the period from January to May, 2020. The prevalence of yeast species were 20%, 22%, 21% and 9% in kareish cheese, pigeon, wild birds and woman vulvovaginal swabs, respectively. The isolated yeast species, mainly Candida albicans species, C. catenulata, C. lusitaniae, C. lipolytica, C.glabrata, C. kefyr, C. guilliermondii, , C. parapsilosis, C. krusei, C. peltata , C. norvegensis C. lambica , C. tropicalis, Cryptococcus neoformans and Rhodotorula rubra depending on their chlamydospore production on rice agar medium and confirmed by germ tube formation. Also we examined the genetic characteristics of the isolated strains by restriction fragment length polymorphism (RFLP) PCR and internal transcribed spacer (ITS). In terms of antifungal activity, fluconazole was effective against most isolates of C. albicans. The isolates of C. albicans and C. catenulate were all resistant to Amphotericin-B, whereas none of the isolates of yeasts were resistant to Nystatin.