Abstract
Any bacterial species is found to be regulating their activities like pathogenicity, antibiotic resistant, biofilm production, and others by phenomenon system called quorum sensing. This system is dependent on production signaling molecules called autoinducers (AI); consequently, we tested the presence of QS genes by polymerase chain reaction (PCR) among different gram negative bacterial pathogens.Material and Methods: Fifty isolates of each of Citrobacter spp., E. coli, Salmonella typhimurium, and Pseudomonas auroginosa were obtained from different private clinical laboratories in Baghdad and Al-Najaf cites in the period of June 2020- February 2021. Bacteria were identified using CHROM agar Orientation followed by Vitek2 system. Quorum sensing genes (abaI 382bp and AHL gene 498bp) were screened by PCR.
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