PCR detection of Gene Stmpr1, Stmpr2 and Stmpr3 encoding protease of Stenotrophomonas malltophilia isolated from different infection

Abstract

Stenotrophomonas maltophilia is an emerging opportunistic nosocomial pathogen, with many virulence factor and causing various infections. Therefore this study conducted to characterization and molecular investigation of some extracellular virulence factor particularly serin protease and detection of gene encoding this factor (Stmpr1, Stmpr2 and Stmpr3 genes) in order to determinants the role of this bacteria in causing infection in Al- Najaf province. Therefore 485 clinical specimens were collected from different infection 315 (64.9%) of specimens were collected from male and 170(35.1%) from female, the specimens were cultured on suitable media and cultivated at 37 C, The results showed that 373(76.9%) gave bacterial growth while 112(23.1%) appeared no growth after identification of bacterial isolate, 20 of isolate were identified Stenotrophomonas maltophilia recovered from the different infections, from those 12 of isolates were recovered from male specimens, while 8 from female, PCR were used to determinant the predominant of protease genes among Stenotrophomona maltophilia isolates, the results of PCR revealed that all isolate 20 (100%) were carried stmpr1, stmpr2 and (85%) Stmpr3 genes, which appeared in positive bands with the correct product size.