PCR detection of Gene lip and plcN1 encoding lipase and lecithinase of Stenotrophomonas malltophilia isolated from different infection

Abstract

Stenotrophomonas maltophilia is a low-virulence opportunistic pathogen that has been identified as an emerging hospital pathogen, Infections caused by S. maltophilia have increased over the years due to increased high-risk patients e.g. patients with immune suppression, hematological malignancies, ICU admission, It is an aerobic, gram- negative, bacilli, motile, non_capsulated and non_ferment glucose, has several virulence potentials for causing various infection. therefore this study conducted to characterization and molecular investigation of some extracellular virulence factor particularly lipase and lecithinase and detection of gene encoding this factors (lip and plcN1genes) in order to determinants the role of this bacteria in causing infection in Al- Najaf province. Therefore 485 clinical specimens were collected from different infection 315(64.9 %) of specimens were collected from male and 170(35.1%) from female, the specimens were cultured on suitable media and cultivated at 37 ℃, The results showed that 373(76.9%) gave bacterial growth while 112(23.1%) appeared no growth. after identification of bacterial isolate, 20 of isolate were identified S. maltophilia recovered from the different infections, from those 12 of isolates were recovered from male specimens, while 8 from female, PCR were used to determinant the predominant of lipase and lecithinase genes among S. maltophilia isolates,,,