Abstract
The covalent attachment of proteins and their patterning on surfaces are important in the bio-medical industry, for use in applications such as bio-sensing and biocompatible medical devices. In this work, proteins are chemisorbed by reaction of their amine groups to glutaraldehyde- and carboxylic acid-functionalised surfaces, within patterns obtained by the dewetting of thin polystyrene films. The proof of principle is shown that consecutive attachment of proteins within the same pattern is possible using this method.
Highlights
The covalent attachment of proteins and their patterning on surfaces are important in the bio-medical industry, for use in applications such as bio-sensing and biocompatible medical devices
The preparation of surface patterns containing proteins has enabled a number of biomedical applications,[1,2] such as the design of biosensors and microarrays, where multiplexing can be used to detect the presence of different types of proteins in a sample mixture.[3,4,5]
Patterning by dewetting is a valid and simple alternative to more sophisticated micro-patterning techniques, such as micro-contact printing, photolithography and micro uidic techniques. This approach has proven to be very effective in producing protein patterns of controlled size and density in a protein-repellent top polymer layer, on top of a protein-adhesive bottom polymer layer
Summary
The covalent attachment of proteins and their patterning on surfaces are important in the bio-medical industry, for use in applications such as bio-sensing and biocompatible medical devices. Proteins are chemisorbed by reaction of their amine groups to glutaraldehyde- and carboxylic acid-functionalised surfaces, within patterns obtained by the dewetting of thin polystyrene films.
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