Abstract
In the present study, the construction of arrays on silicon for naked-eye detection of DNA dengue was demonstrated. The array was created by exposing a polyethylene glycol (PEG) silane monolayer to 254 nm ultraviolet (UV) light through a photomask. Formation of the PEG silane monolayer and photomodifed surface properties was thoroughly characterized by using atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS), and contact angle measurements. The results of XPS confirmed that irradiation of ultraviolet (UV) light generates an aldehyde functional group that offers conjugation sites of amino DNA probe for detection of a specific dengue virus target DNA. Employing a gold enhancement process after inducing the electrostatic interaction between positively charged gold nanoparticles and the negatively charged target DNA hybridized to the DNA capture probe allowed to visualize the array with naked eye. The developed arrays demonstrated excellent performance in diagnosis of dengue with a detection limit as low as 10 pM. The selectivity of DNA arrays was also examined using a single base mismatch and noncomplementary target DNA.
Highlights
Dengue fever, caused by the dengue virus (DENV), is transmitted through the bite of female mosquitoes, mainly of the species of Aedes aegypti and Aedes albopictus [1]
The symptoms normally appear in 3–14 days after the affective bite and primary infection of the virus will cause mild febrile disease, which potentially leads to life-threatening severe dengue and dengue shock syndrom (DSS) should the symptoms are not treated [2,3]
Reverse transcription-polymerase chain reaction (RT-PCR) in clinical serum samples shows that the limit of detection of twofold serial dilution of transcribed RNA varies from 6.0 × to 1.1 × GCE/mL, depending on sample time-point and DENV target
Summary
Dengue fever, caused by the dengue virus (DENV), is transmitted through the bite of female mosquitoes, mainly of the species of Aedes aegypti and Aedes albopictus [1]. RT-PCR in clinical serum samples shows that the limit of detection of twofold serial dilution of transcribed RNA varies from 6.0 × to 1.1 × GCE/mL, depending on sample time-point and DENV target Despite their sensitivity, the tests are tedious, time consuming, involving expensive apparatus and likely to give false-positive reading due to cross contamination with dengue virus PCR product [5,6]. Employed enhancement of daunorubcin conjugated to gold nanoparticles (DNR-AuNPs) which bind the double-stranded DNA on the microarray This method successfully detected a hemagglutinin-subtyping DNA [12]. We presented the fabrication of UV-array of silane surfaces for the label-free dengue detection of synthetic dengue virus oligomers using the enhancement of gold nanoparticles (Figure 1). Close to where it is first mentioned detection of dengue
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