Pattern of Antibody Response to Trypanosoma evansi Infection: A step for production of high affinity antibodies

Abstract

TThe aim of this study is to determine the pattern of immune response during Trypanosoma evansi infection as a preliminary step for production of high affinity antibodies for use in immunoassays. Antibody response to Typanosoma evansi infection in mice was monitored during a short and a long immunization protocols. Both protocols yielded immunoglobulin M (IgM) and immunoglobulin G (IgG) responses. Observed initial antibody response was of an IgM type and later replaced by an IgG response. The IgM response was similar in both immunization protocols. It first detected on day 4, reached a peak level on day 6 and declined thereafter until it disappeared on day 37-post infection. The IgG response in the short protocol was first detected on day 17-post infection, reached a peak level on day 23 and maintained a high level up to the end of the experiment on day 45 post-infection. The IgG response in the long protocol was first detected on day 10-post infection, reached a peak level on day 42 and maintained this level to the end of the experiment. The current study suggests for the first time the pattern of immune response during Trypanosoma evansi infection as a preliminary step for production of high affinity antibodies.